Moraes Joyce L, Moraes Amanda B, Aran Veronica, Alves Marcelo R, Schluckbier Luciene, Duarte Mariana, Toscano Edson, Zamboni Mauro, Sternberg Cinthya, de Moraes Emanuela, Lapa E Silva José R, Ferreira Carlos Gil
Clinical Research Division, Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Evandro Chagas Clinical Research Institute, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.
Mol Clin Oncol. 2017 Apr;6(4):494-502. doi: 10.3892/mco.2017.1167. Epub 2017 Feb 16.
The enzyme cyclooxygenase 2 (COX-2) is known to be involved in tumorigenesis and metastasis in certain types of cancer. Nevertheless, the prognostic value of COX-2 overexpression and its polymorphisms in patients with non-small cell lung cancer (NSCLC) have yet to be fully elucidated. The aim of the present study was to investigate the association between the three most commonly studied COX-2 gene polymorphisms (-1195 G/A, -765 G/C and 8473 T/C) with COX-2 expression and lung cancer risk in a Brazilian cohort. In the present hospital based, case-control retrospective study, 104 patients with NSCLC and 202 cancer free control subjects were genotyped for -1195 G/A, -765 G/C and 8473 T/C polymorphisms using allelic discrimination with a reverse transcription quantitative polymerase chain reaction method. COX-2 mRNA expression was analyzed in surgically resected tumors from 34 patients with NSCLC. The results revealed that COX-2 expression levels were higher in tumor tissue compared with normal lung tissue. However, this overexpression of COX-2 was not associated with the patient outcome, and furthermore, none of the analyzed polymorphisms were associated with the risk of developing lung cancer, COX-2 overexpression, or the overall survival of the patients with NSCLC. Taken together, the findings described in the present study do not support a major role for COX-2 polymorphisms and COX-2 overexpression in lung carcinogenesis within the Brazilian population.
已知环氧合酶2（COX-2）参与某些类型癌症的肿瘤发生和转移。然而，COX-2过表达及其多态性在非小细胞肺癌（NSCLC）患者中的预后价值尚未完全阐明。本研究的目的是在一个巴西队列中研究三种最常研究的COX-2基因多态性（-1195 G/A、-765 G/C和8473 T/C）与COX-2表达及肺癌风险之间的关联。在本基于医院的病例对照回顾性研究中，使用等位基因鉴别和逆转录定量聚合酶链反应方法对104例NSCLC患者和202名无癌对照受试者进行了-1195 G/A、-765 G/C和8473 T/C多态性基因分型。对34例NSCLC患者手术切除的肿瘤组织进行了COX-2 mRNA表达分析。结果显示，与正常肺组织相比，肿瘤组织中COX-2表达水平更高。然而，COX-2的这种过表达与患者预后无关，此外，所分析的多态性均与肺癌发生风险、COX-2过表达或NSCLC患者的总生存期无关。综上所述，本研究中描述的结果不支持COX-2多态性和COX-2过表达在巴西人群肺癌发生中起主要作用。
