Pu Juan, Zhang Guo-zhong, Ma Ji-hong, Xia Ying-ju, Liu Qin-fang, Jiang Zhan-lei, Wang Zhe, Brown Earl G, Tian Fu-lin, Liu Jin-hua
Laboratory of Infectious Diseases, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
Avian Dis. 2009 Jun;53(2):198-204. doi: 10.1637/8410-071708-Reg.1.
H3-subtype influenza viruses are known to infect avian and mammalian species, including humans. However, little is known about the prevalence of H3 influenza virus infection in chicken populations in China. Therefore, a serologic survey of chickens was conducted in China to investigate the seroprevalence of avian H3-subtype influenza virus. Anti-H3 antibodies were assayed by using hemagglutination inhibition (HI) and confirmatory virus neutralization (VN) testing of 4598 serum samples, collected between July 2006 and June 2007, from 173 chicken flocks located in 18 areas that included 16 provinces and two municipalities. Seroepidemiologic results indicated that avian H3-subtype viruses were circulating in chickens in some regions of China, regions that included 12 of the 18 test areas, with an overall average prevalence rate of 2.83%. Samples from 44 of 173 flocks were HI/VN seropositive, including 15 flocks with levels that ranged from 10.00% to 41.94%. Significantly higher seroprevalence rates were observed in older chicken flocks and in those sampled in the cooler seasons. Standardized comparisons showed that Guangdong and Jiangsu, located in the south and east of China, respectively, had significantly higher levels of H3 seropositivity. For the first time, these results demonstrated serologic evidence for H3 avian influenza virus infection in chicken populations in several locations throughout China. These observations highlight the need for continued epidemiologic surveillance of the H3 subtype and for other low-pathogenic avian influenza viruses in China and other regions.
已知H3亚型流感病毒可感染包括人类在内的禽类和哺乳动物。然而，关于中国鸡群中H3流感病毒感染的流行情况知之甚少。因此，在中国对鸡群进行了血清学调查，以研究禽H3亚型流感病毒的血清流行率。通过血凝抑制（HI）试验以及对2006年7月至2007年6月期间从16个省和两个直辖市的18个地区的173个鸡群采集的4598份血清样本进行确证性病毒中和（VN）试验，检测抗H3抗体。血清流行病学结果表明，禽H3亚型病毒在中国部分地区的鸡群中传播，这些地区包括18个检测区域中的12个，总体平均流行率为2.83%。173个鸡群中有44个鸡群的样本HI/VN血清学呈阳性，其中15个鸡群的阳性率在10.00%至41.94%之间。在较老的鸡群以及在较凉爽季节采样的鸡群中观察到显著更高的血清流行率。标准化比较显示，分别位于中国南部和东部的广东和江苏，H3血清阳性水平显著更高。这些结果首次证明了中国多个地区鸡群中存在H3禽流感病毒感染的血清学证据。这些观察结果突出表明，在中国和其他地区需要继续对H3亚型以及其他低致病性禽流感病毒进行流行病学监测。
