Deguchi Kazuhiro, Yokoyama Eriko, Honda Takashi, Mizuno Kyosuke
The Chemo-Sero-Therapeutic Research Institute, 1-6-1 Okubo, Kumamoto 860-8568, Japan.
Avian Dis. 2009 Jun;53(2):281-6. doi: 10.1637/8516-110908-Reg.1.
The safety and efficacy of a novel trivalent inactivated Salmonella vaccine consisting of Salmonella Enteritidis (SE), Salmonella Typhimurium (ST), and Salmonella Infantis (SI) was evaluated with chickens. Chickens were injected subcutaneously with 0.5 ml per dose of the vaccine with an oil adjuvant. To assess the efficacy of the vaccine, vaccinated and unvaccinated chickens were challenged by oral inoculation of SE, ST, SI, and Salmonella Heidelberg (SH) virulent strains 4 wk after vaccination. The vaccinated chickens showed no clinical abnormalities, and the body weight gain after vaccination was comparable to that of unvaccinated chickens. The shedding of SE from the vaccinated chickens at 4, 7, 10, and 14 days postchallenge were significantly reduced in comparison with those of the unvaccinated chickens. The efficacy of the vaccine against ST and SI was comparable to that against SE. The shedding of SH at 4, 7, 10, and 14 days postchallenge was also significantly reduced in the vaccinated chickens compared with those in the unvaccinated suggesting that the vaccine could be effective against different serotype strains having O antigens homologous to those of the vaccine strains. These results suggest that the novel trivalent inactivated vaccine can be an effective tool for controlling the Salmonella infections of O4, O7, and O9 groups in chicken farms.
一种由肠炎沙门氏菌（SE）、鼠伤寒沙门氏菌（ST）和婴儿沙门氏菌（SI）组成的新型三价灭活沙门氏菌疫苗在鸡身上进行了安全性和有效性评估。给鸡皮下注射每剂量0.5毫升含油佐剂的疫苗。为评估疫苗的有效性，在接种疫苗4周后，对接种和未接种疫苗的鸡口服接种SE、ST、SI和海德堡沙门氏菌（SH）强毒株进行攻毒。接种疫苗的鸡未出现临床异常，接种疫苗后的体重增加与未接种疫苗的鸡相当。与未接种疫苗的鸡相比，接种疫苗的鸡在攻毒后第4、7、10和14天SE的排泄量显著减少。该疫苗对ST和SI的有效性与对SE的有效性相当。与未接种疫苗的鸡相比，接种疫苗的鸡在攻毒后第4、7、10和14天SH的排泄量也显著减少，这表明该疫苗可能对具有与疫苗株同源O抗原的不同血清型菌株有效。这些结果表明，这种新型三价灭活疫苗可以成为控制养鸡场中O4、O7和O9组沙门氏菌感染的有效工具。
