Li X, Claesson-Welsh L
Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, Dag Hammarskjöldsv. 20, 751 85 Uppsala, Sweden.
J Thromb Haemost. 2009 Jul;7 Suppl 1:53-6. doi: 10.1111/j.1538-7836.2009.03427.x.
Embryonic stem cells have become an established tool in vascular biology to study the details of vasculogenesis as well as angiogenesis. There is also a future potential in using embryonic stem cell-derived endothelial cells for therapeutic purposes. It is important to evaluate this model by comparing features of endothelial cells derived from differentiating stem cells and their responsiveness to external stimuli to those of primary endothelial cells and to in vivo models. Through culture of mouse embryonic stem cell we discovered that differentiating stem cells are highly amenable to analyzing biochemical and cell biologic processes that are independent of flow. Endothelial cell function can be studied in the context of mutations or deletions that are embryonically lethal in vivo. Many, if not all, of the features of sprouting angiogenesis in differentiating stem cells closely mimic the in vivo process.
胚胎干细胞已成为血管生物学中研究血管生成和血管新生细节的既定工具。利用胚胎干细胞衍生的内皮细胞用于治疗目的也具有未来潜力。通过比较分化干细胞衍生的内皮细胞的特征及其对外部刺激的反应与原代内皮细胞和体内模型的特征来评估该模型很重要。通过小鼠胚胎干细胞培养，我们发现分化干细胞非常适合分析独立于血流的生化和细胞生物学过程。可以在体内具有胚胎致死性的突变或缺失的背景下研究内皮细胞功能。分化干细胞中许多（如果不是全部）发芽血管新生的特征与体内过程非常相似。
