Geurden T, Levecke B, Cacció S M, Visser A, De Groote G, Casaert S, Vercruysse J, Claerebout E
Laboratory of Veterinary Parasitology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Parasitology. 2009 Sep;136(10):1161-8. doi: 10.1017/S0031182009990436. Epub 2009 Jul 27.
Stool samples from Belgian patients suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54.2% and C. parvum in 45.8% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IIaA15G2R1 of C. parvum were the most prevalent, although several other subgenotypes were identified. For Giardia, sequencing at the beta-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74.4%) in human patients. A high degree of heterogeneity was found, especially on the beta-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32.4%) of human patients, which might have important epidemiological implications.
对来自比利时腹痛和/或腹泻患者的粪便样本进行隐孢子虫和贾第虫检测。对隐孢子虫阳性样本使用70 kDa热休克蛋白和60 kDa糖蛋白（GP60）基因进行基因分型：在54.2%的样本中鉴定出人隐孢子虫，在45.8%的样本中鉴定出微小隐孢子虫。GP60位点测序表明，人隐孢子虫的IbA10G2亚型和微小隐孢子虫的IIaA15G2R1亚型最为常见，不过也鉴定出了其他几种亚型。对于贾第虫，β-贾第素、磷酸丙糖异构酶（TPI）和谷氨酸脱氢酶（GDH）基因测序显示，B群在人类患者中最为常见（74.4%）。发现高度的异质性，尤其是在β-贾第素基因上，在GDH基因上的异质性程度较低。此外，使用基于TPI基因的新型种特异性PCR，在大量（32.4%）人类患者中检测到A群和B群的混合感染，这可能具有重要的流行病学意义。
