Influence of the medium electrolyte concentration on the electric polarizability of bacteria Escherichia coli in presence of ethanol.

The electric polarizability is an important parameter of bacteria, giving information about the electric properties of the cells. In our previous works [A.M. Zhivkov, A.Y. Gyurova, Colloids Surf. B: Biointerfaces 66 (2008) 201; A.Y. Gyurova, A.M. Zhivkov, Biophys. Chem., 139 (2009) 8; A.M. Zhivkov, A.Y. Gyurova, J. Phys. Chem. B, 113 (2009) 8375] we have applied an experimental approach to distinguish the contribution of the components of the two types of interface electric polarizability-surface charge dependent (ChD) and Maxwell-Wagner (MW) polarizability. It is based on electro-optical study of the separate influence of the outer and inner medium electrolyte concentration, which changes the external ChD and internal MW components of polarizability; the last effect is reached by the membrane permeability increase in low ethanol concentration. In the present work we investigate the behavior of electric polarizability of Escherichia coli K12 at increasing the outer KCl concentration in presence of 10 vol.% ethanol in order to check if the polarizability components change independently from one another. The conclusion is that the outer electrolyte concentration influence indirectly the internal MW component by the trans-membrane concentration gradient, but the polarizability components themselves change independently.