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[表面活性剂对细菌细胞电特性的影响]

[Effect of surface-active agents on the electrical properties of bacterial cells].

作者信息

Fomchenkov V M, Ivanov A Iu, Azhermachev A K, Chugunov V A, Miroshnikov A I

出版信息

Mikrobiologiia. 1986 Jul-Aug;55(4):601-6.

PMID:3095615
Abstract

The work was aimed at studying the effect of cationic, anionic and non-ionogenic surfactants on the frequency dependence of the electroorientation effect (EOE) and on the electrophoretic mobility (EPM) of rod-like bacteria. The character of concentration dependences was found to differ for EOE and EPM at a low frequency of the electric field (20 to 10(4) Hz). Analysis of EOE changes at a high frequency (4 X 10(5) to 3 X 10(7) Hz) showed that anionic and non-ionogenic surfactants at a concentration up to 10(-3) M did not damage Escherichia coli, a Gram-negative bacterium, in contrast to Bacillus cereus, a Gram-positive bacterium. Cationic surfactants affected the cells of the both species. The optical properties of bacterial cells were found to change under the action of cationic surfactants.

摘要

该研究旨在探讨阳离子、阴离子和非离子表面活性剂对棒状细菌的电取向效应(EOE)频率依赖性以及电泳迁移率(EPM)的影响。研究发现,在低频电场(20至10⁴Hz)下,EOE和EPM的浓度依赖性特征有所不同。对高频(4×10⁵至3×10⁷Hz)下EOE变化的分析表明,浓度高达10⁻³M的阴离子和非离子表面活性剂不会损伤革兰氏阴性菌大肠杆菌,这与革兰氏阳性菌蜡样芽孢杆菌不同。阳离子表面活性剂会影响这两种细菌的细胞。研究发现,在阳离子表面活性剂的作用下,细菌细胞的光学性质会发生变化。

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