Kalili Kathithileni M, de Villiers André
Stellenbosch University, Department of Chemistry and Polymer Science, Private Bag X1, Matieland 7602, South Africa.
J Chromatogr A. 2009 Aug 28;1216(35):6274-84. doi: 10.1016/j.chroma.2009.06.071. Epub 2009 Jul 1.
The development of an off-line comprehensive 2-dimensional liquid chromatography (2-D-LC) method for the analysis of procyanidins is reported. In the first dimension, oligomeric procyanidins were separated according to molecular weight by hydrophilic interaction chromatography (HILIC), while reversed phase LC was employed in the second dimension to separate oligomers based on hydrophobicity. Fluorescence, UV and electrospray ionisation mass spectrometry (ESI-MS) were employed for identification purposes. The combination of these orthogonal separation methods is shown to represent a significant improvement compared to 1-dimensional methods for the analysis of complex high molecular weight procyanidin fractions, by simultaneously providing isomeric and molecular weight information. The low correlation (r(2)<0.2100) between the two LC modes afforded a practical peak capacity in excess of 2300 for the optimal off-line method. The applicability of the method is demonstrated for the analysis of phenolic extracts of apple and cocoa.
报道了一种用于分析原花青素的离线综合二维液相色谱（2-D-LC）方法的开发。在第一维中，通过亲水相互作用色谱（HILIC）根据分子量分离低聚原花青素，而在第二维中采用反相LC基于疏水性分离低聚物。采用荧光、紫外和电喷雾电离质谱（ESI-MS）进行鉴定。与一维方法相比，这些正交分离方法的组合在分析复杂的高分子量原花青素组分时，通过同时提供异构体和分子量信息，显示出显著的改进。两种LC模式之间的低相关性（r(2)<0.2100）为最佳离线方法提供了超过2300的实际峰容量。该方法的适用性在苹果和可可酚提取物的分析中得到了证明。
