Majdoub Hatem, Ben Mansour Mohamed, Chaubet Frédéric, Roudesli Mohamed S, Maaroufi Raoui M
Laboratoire Polymères, Biopolymères et Matériaux Organiques, Faculté des Sciences, Monastir, 5000 Monastir, Tunisia.
Biochim Biophys Acta. 2009 Oct;1790(10):1377-81. doi: 10.1016/j.bbagen.2009.07.013. Epub 2009 Jul 23.
The polysaccharide of culture medium from Arthrospira platensis was extracted by ultrafiltration, partially characterized and assayed for anticoagulant activity.
The crude polysaccharidic fraction was fractionated by anion exchange chromatography on DEAE-cellulose, subjected to acetate cellulose electrophoresis and characterized by physicochemical procedures. The anticoagulant effect of the ultrafiltrated polysaccharide was checked by several coagulation tests.
Anion exchange chromatography revealed in the whole ultrafiltrated polysaccharidic fraction the occurrence of a sulfated spirulan-like component designated PUF2. The average molecular weight of PUF2 was determined by size exclusion chromatography combined with multi-angle light scattering (SEC-MALS) and viscosimetry and was 199 kDa and the sulfate content was 20% weight/dry weight. The physicochemical characterization indicated the occurrence of rhamnose (49.7%), galacturonic and glucuronic acid (32% of total sugar). The anticoagulant effect of this sulfated polysaccharide was mainly due to the potentiation of thrombin inhibition by heparin cofactor II and was 4-times higher than that of the porcine dermatan sulfate whereas it had no effect on anti-Xa activity.
An ultrafiltrated sulfated polysaccharide, likely a calcium spirulan was obtained from the culture medium of A. platensis and showed an anticoagulant activity mediated by heparin cofactor II.
Old culture medium of A. platensis may represent an important source for the spirulan-like PUF2 which was endowed with potentially useful anticoagulant properties whereas its obtention by ultrafiltration may represent an extraction procedure of interest.
采用超滤法从钝顶螺旋藻培养基中提取多糖，对其进行部分表征并检测抗凝活性。
粗多糖级分通过DEAE - 纤维素阴离子交换色谱进行分级分离，进行醋酸纤维素电泳，并采用物理化学方法进行表征。通过多种凝血试验检测超滤多糖的抗凝效果。
阴离子交换色谱显示，在整个超滤多糖级分中存在一种硫酸化的类螺旋藻多糖成分，命名为PUF2。通过尺寸排阻色谱结合多角度光散射（SEC - MALS）和粘度测定法测定PUF2的平均分子量为199 kDa，硫酸含量为20%（重量/干重）。物理化学表征表明存在鼠李糖（49.7%）、半乳糖醛酸和葡萄糖醛酸（占总糖的32%）。这种硫酸化多糖的抗凝作用主要归因于增强了肝素辅因子II对凝血酶的抑制作用，其抗凝效果比猪硫酸皮肤素高4倍，而对Xa因子活性无影响。
从钝顶螺旋藻培养基中获得了一种超滤硫酸化多糖，可能是钙螺旋藻多糖，其显示出由肝素辅因子II介导的抗凝活性。
钝顶螺旋藻的旧培养基可能是类螺旋藻多糖PUF2的重要来源，该多糖具有潜在有用的抗凝特性，而通过超滤获得该多糖可能是一种有意义的提取方法。
