College of Food Science and Technology, Ocean University of China, 5 Yu Shan Road, Qingdao, Shandong 266003, China.
Int J Biol Macromol. 2011 Dec 1;49(5):1160-6. doi: 10.1016/j.ijbiomac.2011.09.017. Epub 2011 Sep 29.
A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that AAP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14:2.37:2.94:1, the content of sulfate was 15.5%. The average molecular weight (M(w)) was 56.2 kDa in a TSK G4000 column. Further IR, 1D, 2D NMR spectroscopic analysis of the correlation signals of different sugar units gave a proposal repeating structure, as follows: [chemical structure: see the text] In vitro anticoagulant assay indicated AAP prolonged both the activated partial thromboplastin time (APTT) and thrombin time (TT), with a 22.5 U/mg and 72.0 U/mg compared with standard heparin, respectively. The anticoagulant property of AAP was mainly attributed to powerful potentiation thrombin by anti-thrombin III.
一种新型糖胺聚糖样硫酸多糖（AAP）从鲍鱼的腹足中分离纯化。Haliotis discus hannai Ino 通过 DEAE 离子交换色谱和 S-300 HR 凝胶柱色谱进行分离纯化。化学组成分析表明 AAP 由氨基半乳糖、葡萄糖醛酸、岩藻糖、半乳糖组成，摩尔比为 2.14:2.37:2.94:1，硫酸根含量为 15.5%。在 TSK G4000 柱上的重均分子量（Mw）为 56.2 kDa。进一步的红外、1D、2D NMR 相关信号分析表明不同糖单元的重复结构如下：[化学结构：见正文]体外抗凝血实验表明 AAP 能延长活化部分凝血活酶时间（APTT）和凝血酶时间（TT），分别为 22.5 U/mg 和 72.0 U/mg，与标准肝素相比。AAP 的抗凝特性主要归因于抗凝血酶 III 对凝血酶的强大增强作用。
