University of Ottawa Eye Institute, Ottawa, Ont., Canada K1H 8L6.
Acta Biomater. 2010 Jan;6(1):187-94. doi: 10.1016/j.actbio.2009.07.027. Epub 2009 Jul 24.
6-Methacryloyl-alpha-D-galactopyranose (MG) was synthesized, and characterized by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectrometry, and single-crystal X-ray diffraction. A series of interpenetrating polymer network (IPN) hydrogels was fabricated by simultaneously photocuring MG crosslinked by poly(ethylene glycol) diacrylate and chemically crosslinking type I collagen with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The successful incorporation of the glycopolymer, polymer MG, into collagen hydrogel was confirmed by FTIR and solid-state (13)C NMR. The optical characteristics of the IPN hydrogels are comparable to those of human corneas. The tensile strength and modulus of the hydrogels are enhanced by incorporation of polymer MG in comparison to that of the control collagen hydrogel. Biodegradation results indicated that polymer MG enhanced the stability of the composite hydrogels against collagenase. In vitro results demonstrated that the IPN hydrogel supported the adhesion and proliferation of human corneal epithelial cells and outperformed human cornea in blocking bacteria adhesion. Taken together, the IPN hydrogel might be a promising material for use in corneal lamellar keratoplasty.
6-甲基丙烯酰基-α-D-吡喃半乳糖（MG）被合成，并通过傅里叶变换红外（FTIR）和核磁共振（NMR）光谱以及单晶 X 射线衍射进行了表征。通过同时光固化由聚乙二醇二丙烯酸酯交联的 MG 以及用 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺和 N-羟基琥珀酰亚胺化学交联 I 型胶原，制备了一系列互穿聚合物网络（IPN）水凝胶。通过 FTIR 和固态（13）C NMR 证实了糖聚合物聚合物 MG 成功掺入胶原水凝胶中。IPN 水凝胶的光学特性与人类角膜相当。与对照胶原水凝胶相比，掺入聚合物 MG 可提高水凝胶的拉伸强度和模量。降解结果表明，聚合物 MG 增强了复合水凝胶对胶原酶的稳定性。体外结果表明，IPN 水凝胶支持人角膜上皮细胞的黏附和增殖，并且在阻止细菌黏附方面优于人角膜。综上所述，IPN 水凝胶可能是用于角膜层状角膜移植的有前途的材料。
