Cai Guohong, Myers Kevin, Hillman Bradley I, Fry William E
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
Virology. 2009 Sep 15;392(1):52-61. doi: 10.1016/j.virol.2009.06.040. Epub 2009 Jul 25.
Double-stranded RNA representing four distinct electrophoretic patterns was found in a screen of Phytophthora infestans isolates. Two dsRNAs that always appeared together were sequenced. RNA 1, which was 3160 nt plus a poly (A) tail, contained a single deduced ORF with the potential to encode a polyprotein of 977 aa with motifs characteristic of supergroup I viral RdRps. The 2776 nt, polyadenylated RNA2 contained an ORF with a potential to encode a polyprotein of 847 aa including a possible trypsin-like serine protease, and a second putative ORF of unknown function. An alternative form of RNA2, in which a 19-nt stretch was replaced by a 9-nt sequence, was detected in 4 of 17 clones sequenced. Based on genome structure and phylogenetic analysis, this virus did not fit into any known virus family and we tentatively named it Phytophthora infestans RNA virus 1 (PiRV-1).
在对致病疫霉分离株的筛选中发现了代表四种不同电泳模式的双链RNA。对总是一起出现的两种双链RNA进行了测序。RNA 1长3160 nt，带有一个聚腺苷酸尾巴，包含一个单一的推导开放阅读框，有可能编码一个977个氨基酸的多聚蛋白，具有超群I病毒RNA依赖性RNA聚合酶（RdRps）的特征基序。2776 nt的聚腺苷酸化RNA2包含一个开放阅读框，有可能编码一个847个氨基酸的多聚蛋白，包括一个可能的胰蛋白酶样丝氨酸蛋白酶，以及一个功能未知的第二个推定开放阅读框。在17个测序克隆中的4个中检测到RNA2的一种替代形式，其中一段19 nt的序列被一段9 nt的序列取代。基于基因组结构和系统发育分析，这种病毒不属于任何已知的病毒科，我们暂时将其命名为致病疫霉RNA病毒1（PiRV-1）。
