Cai Guohong, Myers Kevin, Fry William E, Hillman Bradley I
Crop production and Pest Control Research Unit, ARS, USDA and Botany and Plant Pathology Department, Purdue University, West Lafayette, IN, USA.
School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
Arch Virol. 2019 Feb;164(2):567-572. doi: 10.1007/s00705-018-4050-0. Epub 2018 Oct 20.
Phytophthora infestans is the causal agent of potato and tomato late blight. In this study, we obtained the complete genome sequence of a novel RNA virus from this plant pathogen, tentatively named "Phytophthora infestans RNA virus 2" (PiRV-2). The PiRV-2 genome is 11,170 nt in length and lacks a polyA tail. It contains a single large open reading frame (ORF) with short 5' and 3' untranslated regions. The ORF is predicted to encode a polyprotein of 3710 aa (calculated molecular weight, 410.94 kDa). This virus lacks significant similarity to any other known viruses, even in the conserved RNA-dependent RNA polymerase region. Phylogenetic analysis demonstrated that it did not cluster with any known virus group. We conclude that PiRV-2 belongs to a new virus family yet to be described. This virus was found to be faithfully transmitted through asexual reproduction.
致病疫霉是马铃薯和番茄晚疫病的病原体。在本研究中，我们从这种植物病原体中获得了一种新型RNA病毒的完整基因组序列，暂命名为“致病疫霉RNA病毒2”（PiRV-2）。PiRV-2基因组长度为11,170 nt，无polyA尾。它包含一个单一的大开放阅读框（ORF），5'和3'非翻译区较短。该ORF预计编码一个3710个氨基酸的多聚蛋白（计算分子量为410.94 kDa）。这种病毒与任何其他已知病毒均无显著相似性，即使在保守的RNA依赖RNA聚合酶区域也是如此。系统发育分析表明，它不与任何已知病毒群聚类。我们得出结论，PiRV-2属于一个尚未描述的新病毒科。发现这种病毒可通过无性繁殖稳定传播。
