State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640 Guangdong, China.
J Hazard Mater. 2009 Nov 15;171(1-3):948-53. doi: 10.1016/j.jhazmat.2009.06.100. Epub 2009 Jun 25.
Batch experiments were conducted on the degradation of synthetic and municipal wastewater by six different strains, i.e., Agrobacterium sp., Bacillus sp., Enterobacter cloacae, Gordonia, Pseudomonas stutzeri, Pseudomonas putida. By applying a fractional factorial design (FFD) of experiments, the influence of each strain and their interactions were quantified. An empirical model predicting the treatment efficiency was built based on the results of the FFD experiments with an R(2) value of 99.39%. For single strain, Enterobacter cloacae, Gordonia and P. putida (p=0.008, 0.009 and 0.023, respectively) showed significant enhancement on organic removal in the synthetic wastewater. Positive interaction from Enterobacter cloacae, Gordonia (p=0.046) was found, indicating that syntrophic interaction existed, and their coexistence can improve total organic carbon (TOC) degradation. Verification experiments were performed to evaluate the effect of bioaugmentation by introducing three selected strains into an activated sludge reactor for treating municipal wastewater. The removal efficiency of TOC with the bioaugmentation was increased from 67-72% to 80-84% at an influent TOC concentration of 200mg/L. The results derived from this study indicate that the FFD is a useful screening tool for optimizing the microbial community to enhance treatment efficiency.
采用六株不同的菌株（即根癌农杆菌、芽孢杆菌、阴沟肠杆菌、戈登氏菌、施氏假单胞菌和恶臭假单胞菌）进行了降解合成废水和城市废水的批实验。通过应用实验的部分因子设计（FFD），量化了每种菌株及其相互作用的影响。根据 FFD 实验的结果，建立了一个预测处理效率的经验模型，其 R(2)值为 99.39%。对于单一菌株，阴沟肠杆菌、戈登氏菌和恶臭假单胞菌（p=0.008、0.009 和 0.023）在合成废水中对有机物去除表现出显著的增强作用。发现阴沟肠杆菌、戈登氏菌存在正相互作用（p=0.046），表明存在共生相互作用，它们的共存可以提高总有机碳（TOC）的降解。通过引入三株选定的菌株到活性污泥反应器中处理城市废水，进行了生物强化的验证实验。在进水 TOC 浓度为 200mg/L 时，生物强化的 TOC 去除效率从 67-72%提高到 80-84%。本研究的结果表明，FFD 是优化微生物群落以提高处理效率的有用筛选工具。
