Kaur M, Sood M L
Department of Zoology, Punjab Argricultural University, Ludhiana, India.
Angew Parasitol. 1990 Aug;31(3):167-81.
Haemonchus contortus, incubated in 10 micrograms/ml and 50 micrograms/ml concentrations of Nilzan and albendazole in Tyrode solution were stained for histoenzymatic demonstration of various phosphatases, oxido-reductases and esterases. The intestine showed major alterations after drug treatments. The alkaline phosphatases (AkPase), adenosine triphosphatase (ATPase), glucose-6-phosphatase, succinic dehydrogenase (SDH), glutamate dehydrogenase (GDH), reduced nicotinamide adenine dinucleotide phosphate diaphorase and reduced nicotinamide adenine dinucleotide diaphorase showed a decreased activity in intestine after Nilzan treatment, whereas lactic dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PD) and monoamine oxidase resisted increased reaction. The albendazole treatment resulted in altered distribution pattern of the AkPase, ATPase, SDH, and GDH; while LDH, G-6-PD, and non-specific esterases exhibited slightly enhanced activity in the epithelium. The functional significance of these changes has been fully discussed.
将捻转血矛线虫置于含有10微克/毫升和50微克/毫升尼立达唑及阿苯达唑的台氏液中孵育，然后对其进行染色，以组织酶法显示各种磷酸酶、氧化还原酶和酯酶。药物处理后，肠道出现了主要变化。尼立达唑处理后，肠道中的碱性磷酸酶（AkPase）、三磷酸腺苷酶（ATPase）、葡萄糖-6-磷酸酶、琥珀酸脱氢酶（SDH）、谷氨酸脱氢酶（GDH）、还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶和还原型烟酰胺腺嘌呤二核苷酸黄递酶活性降低，而乳酸脱氢酶（LDH）、葡萄糖-6-磷酸脱氢酶（G-6-PD）和单胺氧化酶的反应增强。阿苯达唑处理导致AkPase、ATPase、SDH和GDH的分布模式改变；而LDH、G-6-PD和非特异性酯酶在上皮细胞中的活性略有增强。已充分讨论了这些变化的功能意义。
