Kaur M, Sood M L
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
Appl Parasitol. 1996 Dec;37(4):302-11.
In vitro incubation of Haemonchus contortus (RUD., 1803) and Trichuris globulosa (v. LINSTOW, 1901) were proformed for 10-12 h in Tyrode's solution and 10 and 50 micrograms/ml concentrations each of albendazole (ABZ), fenbendazole (FBZ), thiophenate (TP), dl-tetramisole and oxyclozanide (TO), dl-tetramisole HCI (TMS) and levamisole HCI (LMS) to study morphological and histochemical alterations. The major structural changes observed after treatment with all the drugs were vacuolation in the intestine of H. contortus except with TP treatment and disruption of the epithelium in T. globulosa except with TMS treatment. The other major alteration in T. globulosa was the loss of muscle striations after TP, TO, TMS and LMS treatments. All the six anthelmintics reduced the quantity of neutral mucopolysaccharides in the intestine of H. contortus and T. globulosa except with TMS and LMS treatments in the latter. Acidic mucopolysaccharides detected in the microvilli of the intestine of H. contortus were lost after TO and ABZ treatments. The loss of lipids from the intestine was evident after TO, ABZ, FBZ and TP treatments in H. contortus and by all the drugs in T. globulosa. LMS treatment caused accumulation of very large lipid droplets in the intestine of H. contortus.
将捻转血矛线虫（鲁德，1803年）和球形鞭虫（林斯托夫，1901年）在泰罗德溶液中，以及在阿苯达唑（ABZ）、芬苯达唑（FBZ）、硫苯咪唑（TP）、消旋四咪唑和氯硝柳胺（TO）、盐酸消旋四咪唑（TMS）和盐酸左旋咪唑（LMS）各自浓度为10和50微克/毫升的溶液中进行10 - 12小时的体外培养，以研究形态学和组织化学变化。在用所有药物处理后观察到的主要结构变化是，除TP处理外，捻转血矛线虫肠道出现空泡化；除TMS处理外，球形鞭虫上皮细胞遭到破坏。球形鞭虫的另一个主要变化是，在TP、TO、TMS和LMS处理后肌肉条纹消失。除TMS和LMS处理后者外，所有六种驱虫药均减少了捻转血矛线虫和球形鞭虫肠道中的中性粘多糖数量。在TO和ABZ处理后，捻转血矛线虫肠道微绒毛中检测到的酸性粘多糖消失。在捻转血矛线虫中，TO、ABZ、FBZ和TP处理后，肠道脂质明显流失；在球形鞭虫中，所有药物处理后均出现这种情况。LMS处理导致捻转血矛线虫肠道中积累非常大的脂滴。
