Simon-Colin C, Raguénès G, Cozien J, Guezennec J G
Institut Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, BIOMAR/BMM, Plouzané, France.
J Appl Microbiol. 2008 May;104(5):1425-32. doi: 10.1111/j.1365-2672.2007.03667.x. Epub 2007 Dec 20.
The objective of the present work was to describe a new deep-sea, aerobic, mesophilic and heterotrophic bacterium, referenced as strain AT1214, able to produce polyhydroxyalkanoates (PHAs) under laboratory conditions. This bacterium was isolated from a shrimp collected nearby a hydrothermal vent located on the Mid-Atlantic Ridge.
This micro-organism, on the basis of the phenotypical features and genotypic investigations, can be clearly assigned to the Halomonas genus and the name of Halomonas profundus is proposed. Optimal growth occurred between 32 and 37 degrees C at a pH between 8 and 9 and at ionic strength between 20 and 30 g l(-1) of sea salts. The G + C content of DNA was 58.6%. This bacterium produced PHAs of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from different carbon sources.
The bacterium H. profundus produces PHA of 3HB and 3HV monomers from different carbon sources.
PHAs share physical and material properties that suggest them for application in various areas, and are considered as an alternative to nonbiodegradable plastics produced from fossil oils. In this study, we describe a new bacteria isolated from a deep-sea hydrothermal vent with the capability to produce polyesters of biotechnological interest.
本研究的目的是描述一种新的深海需氧嗜温异养细菌，编号为AT1214菌株，该菌株在实验室条件下能够产生聚羟基脂肪酸酯（PHA）。此细菌是从采集于大西洋中脊一处热液喷口附近的一只虾类中分离得到的。
基于表型特征和基因研究，这种微生物可明确归为嗜盐单胞菌属，因此提出将其命名为深海嗜盐单胞菌。在32至37摄氏度、pH值为8至9以及离子强度为20至30 g l(-1)海盐的条件下生长最佳。DNA的G + C含量为58.6%。该细菌能利用不同碳源产生聚（3-羟基丁酸酯）和聚（3-羟基丁酸酯-co-3-羟基戊酸酯）的PHA。
深海嗜盐单胞菌能利用不同碳源产生由3HB和3HV单体组成的PHA。
PHA具有的物理和材料特性表明其可应用于各个领域，并且被视为化石油生产的不可生物降解塑料的替代品。在本研究中，我们描述了一种从深海热液喷口分离出的具有生产具有生物技术意义的聚酯能力的新细菌。
