硫胺素、硫胺素磷酸盐及硫胺素代谢酶在大鼠脑神经元和胶质细胞富集组分中的分布
Distribution of thiamine, thiamine phosphates, and thiamine metabolizing enzymes in neuronal and glial cell enriched fractions of rat brain.
作者信息
Laforenza U, Patrini C, Rindi G
机构信息
Institute of Human Physiology, University of Pavia, Italy.
出版信息
J Neurochem. 1988 Sep;51(3):730-5. doi: 10.1111/j.1471-4159.1988.tb01805.x.
The distribution of thiamine, thiamine phosphoesters, and the thiamine pyrophosphate synthetizing [thiamine-pyrophosphokinase (TPKase)] as well as hydrolyzing [thiamine pyrophosphatase (TPPase) and thiamine monophosphatase (TMPase)] enzymes was determined in neuronal and glial enriched fractions prepared from rat brain. Nucleoside diphosphatases [inosine diphosphatase (IDPase) and uridine diphosphatase (UDPase)] and nucleoside monophosphatases [uridine monophosphatase (UMPase) and inosine monophosphatase (IMPase)] were also determined. Thiamine and thiamine mono- and pyrophosphate were present in neuronal enriched fractions at concentrations 2.8, 3.6, and 4.6 times higher than in glial fractions. TMPase was found only in glial enriched fractions, whereas the levels of TPKase, UMPase, IMPase, IDPase, UDPase, and TPPase were 2.0-, 2.2-, 1.3-, 2.8-, 3.7-, and 20.8-fold higher in neuronal than in glial fractions.
在从大鼠脑制备的富含神经元和胶质细胞的组分中，测定了硫胺素、硫胺素磷酸酯、硫胺素焦磷酸合成酶[硫胺素焦磷酸激酶(TPKase)]以及水解酶[硫胺素焦磷酸酶(TPPase)和硫胺素单磷酸酶(TMPase)]的分布。还测定了核苷二磷酸酶[肌苷二磷酸酶(IDPase)和尿苷二磷酸酶(UDPase)]以及核苷单磷酸酶[尿苷单磷酸酶(UMPase)和肌苷单磷酸酶(IMPase)]。硫胺素、硫胺素单磷酸和硫胺素焦磷酸在富含神经元的组分中的浓度分别比在胶质细胞组分中高2.8倍、3.6倍和4.6倍。仅在富含胶质细胞的组分中发现了TMPase，而神经元组分中TPKase、UMPase、IMPase、IDPase、UDPase和TPPase的水平分别比胶质细胞组分高2.0倍、2.2倍、1.3倍、2.8倍、3.7倍和20.8倍。
Zotero 插件