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本文引用的文献

1
Development of fluorescent in situ hybridization for Cryptosporidium detection reveals zoonotic and anthroponotic transmission of sporadic cryptosporidiosis in Sydney.用于隐孢子虫检测的荧光原位杂交技术的发展揭示了悉尼散发性隐孢子虫病的人畜共患和人传人传播情况。
J Microbiol Methods. 2008 Dec;75(3):535-9. doi: 10.1016/j.mimet.2008.08.007. Epub 2008 Aug 27.
2
Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission.隐孢子虫和贾第虫的物种及基因型的分子特征分析与人畜共患病传播评估。
Int J Parasitol. 2008 Sep;38(11):1239-55. doi: 10.1016/j.ijpara.2008.03.006. Epub 2008 Apr 7.
3
Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques.通过改进的荧光原位杂交技术对微生物群落中的单细胞进行鉴定。
Nat Rev Microbiol. 2008 May;6(5):339-48. doi: 10.1038/nrmicro1888.
4
Advances in the epidemiology, diagnosis and treatment of cryptosporidiosis.隐孢子虫病的流行病学、诊断及治疗进展
Expert Rev Anti Infect Ther. 2006 Jun;4(3):429-43. doi: 10.1586/14787210.4.3.429.
5
Mixed Cryptosporidium infections and HIV.隐孢子虫混合感染与艾滋病病毒
Emerg Infect Dis. 2006 Jun;12(6):1025-8. doi: 10.3201/eid1206.060015.
6
Molecular epidemiology of human cryptosporidiosis.人类隐孢子虫病的分子流行病学
Parassitologia. 2005 Jun;47(2):185-92.
7
Environmental inactivation of Cryptosporidium oocysts in catchment soils.隐孢子虫卵囊在集水区土壤中的环境失活
J Appl Microbiol. 2005;98(2):308-17. doi: 10.1111/j.1365-2672.2004.02459.x.
8
Cryptosporidiosis: an update in molecular epidemiology.隐孢子虫病：分子流行病学的最新进展
Curr Opin Infect Dis. 2004 Oct;17(5):483-90. doi: 10.1097/00001432-200410000-00014.
9
Cryptosporidium species and genotypes in HIV-positive patients in Lima, Peru.秘鲁利马艾滋病病毒阳性患者中的隐孢子虫种类和基因型
J Eukaryot Microbiol. 2003;50 Suppl:531-3. doi: 10.1111/j.1550-7408.2003.tb00620.x.
10
Zoonotic species of Cryptosporidium are as prevalent as the anthroponotic in HIV-infected patients in Thailand.
Ann Trop Med Parasitol. 2002 Dec;96(8):797-802. doi: 10.1179/000349802125002202.

用于人类感染性隐孢子虫物种水平鉴定的双色荧光原位杂交技术的开发

Development of a two-color fluorescence in situ hybridization technique for species-level identification of human-infectious Cryptosporidium spp.

作者信息

Alagappan A, Bergquist P L, Ferrari B C

机构信息

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.

出版信息

Appl Environ Microbiol. 2009 Sep;75(18):5996-8. doi: 10.1128/AEM.00643-09. Epub 2009 Jul 24.

DOI:10.1128/AEM.00643-09
PMID:19633112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2747856/
Abstract

A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

摘要

开发了一种双色荧光原位杂交检测方法，可同时鉴定微小隐孢子虫和人隐孢子虫。该检测方法是一种简单、快速且经济高效的工具，用于检测对公共卫生至关重要的主要隐孢子虫种类。