Dun N J, Kaibara K, Karczmar A G
Science. 1977 Aug 19;197(4305):778-80. doi: 10.1126/science.196332.
Acetylcholine (ACh), dopamine, and dibutyryl-adenosine 3',5'-monophosphate (dbcAMP) were applied iontophoretically to the rabbit superior cervical ganglion cells from triple-barreled micropipettes, and the response was recorded by intracellular techniques. All ganglion cells tested responded to the depolarizing action of ACh, whereas less than half of the cells that responded to ACh were hyperpolarized by dopamine. This effect was blocked by low concentrations of haloperidol. None of the cells examined responded to dbcAMP applied by iontophoresis. Hence, the present result is not consistent with the concept that a cyclic AMP mechanism underlies the hyperpolarizing effect of dopamine.
通过三管微量移液器将乙酰胆碱（ACh）、多巴胺和二丁酰腺苷3'，5'-单磷酸（dbcAMP）离子导入家兔颈上神经节细胞，并采用细胞内技术记录反应。所有测试的神经节细胞对ACh的去极化作用均有反应，而对ACh有反应的细胞中，不到一半的细胞会被多巴胺超极化。这种效应被低浓度的氟哌啶醇阻断。经离子导入施加的dbcAMP，未检测到任何细胞有反应。因此，目前的结果与多巴胺超极化作用基于环磷酸腺苷机制这一概念不一致。
