Ruiz Natividad, Kahne Daniel, Silhavy Thomas J
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
Nat Rev Microbiol. 2009 Sep;7(9):677-83. doi: 10.1038/nrmicro2184. Epub 2009 Jul 27.
Intracellular lipid transport is poorly understood. Genetic studies to identify lipid-transport factors are complicated by the essentiality of many lipids, whereas biochemical and cell biology approaches aiming to determine localization and mechanisms of lipid transport are often challenged by the lack of adequate technology. Here, we review the epic history of how different approaches, technological advances and ingenuity contributed to the recent discovery of a multi-protein pathway that transports lipopolysaccharide across the envelope of Gram-negative bacteria.
细胞内脂质转运的相关机制尚不清楚。由于许多脂质的不可或缺性，通过基因研究来鉴定脂质转运因子变得复杂，而旨在确定脂质转运定位和机制的生化及细胞生物学方法，常常因缺乏足够的技术而受到挑战。在此，我们回顾了一段波澜壮阔的历史，讲述了不同方法、技术进步以及智慧如何共同促成了近期对一条多蛋白途径的发现，该途径可将脂多糖转运穿过革兰氏阴性菌的包膜。
