• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脂多糖转运（Lpt）机制：革兰氏阴性菌外膜上用于脂多糖组装的一种非常规转运体。

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria.

作者信息

Sperandeo Paola, Martorana Alessandra M, Polissi Alessandra

机构信息

From the Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, 20133 Milan and.

the Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, 20126 Milan, Italy.

出版信息

J Biol Chem. 2017 Nov 3;292(44):17981-17990. doi: 10.1074/jbc.R117.802512. Epub 2017 Sep 6.

DOI:10.1074/jbc.R117.802512
PMID:28878019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5672026/
Abstract

The outer membrane (OM) of Gram-negative is a unique lipid bilayer containing LPS in its outer leaflet. Because of the presence of amphipathic LPS molecules, the OM behaves as an effective permeability barrier that makes Gram-negative bacteria inherently resistant to many antibiotics. This review focuses on LPS biogenesis and discusses recent advances that have contributed to our understanding of how this complex molecule is transported across the cellular envelope and is assembled at the OM outer leaflet. Clearly, this knowledge represents an important platform for the development of novel therapeutic options to manage Gram-negative infections.

摘要

革兰氏阴性菌的外膜（OM）是一种独特的脂质双层膜，其外层小叶含有脂多糖（LPS）。由于两亲性LPS分子的存在，外膜表现为一种有效的渗透屏障，使革兰氏阴性菌天然地对许多抗生素具有抗性。本综述聚焦于LPS的生物合成，并讨论了最近的进展，这些进展有助于我们理解这种复杂分子是如何穿过细胞包膜并在外膜外层小叶组装的。显然，这些知识是开发治疗革兰氏阴性菌感染新疗法的重要平台。

相似文献

1
The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria.脂多糖转运（Lpt）机制：革兰氏阴性菌外膜上用于脂多糖组装的一种非常规转运体。
J Biol Chem. 2017 Nov 3;292(44):17981-17990. doi: 10.1074/jbc.R117.802512. Epub 2017 Sep 6.
2
Lipopolysaccharide biogenesis and transport at the outer membrane of Gram-negative bacteria.革兰氏阴性菌外膜上的脂多糖生物发生和运输。
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Nov;1862(11):1451-1460. doi: 10.1016/j.bbalip.2016.10.006. Epub 2016 Oct 17.
3
Structural insight into lipopolysaccharide transport from the Gram-negative bacterial inner membrane to the outer membrane.从革兰氏阴性细菌内膜到外膜的脂多糖转运的结构见解。
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Nov;1862(11):1461-1467. doi: 10.1016/j.bbalip.2017.08.003. Epub 2017 Aug 15.
4
The Lpt ABC transporter for lipopolysaccharide export to the cell surface.Lpt ABC 转运体将脂多糖输出到细胞表面。
Res Microbiol. 2019 Nov-Dec;170(8):366-373. doi: 10.1016/j.resmic.2019.07.005. Epub 2019 Aug 1.
5
Combining Mutations That Inhibit Two Distinct Steps of the ATP Hydrolysis Cycle Restores Wild-Type Function in the Lipopolysaccharide Transporter and Shows that ATP Binding Triggers Transport.结合抑制 ATP 水解循环两个不同步骤的突变可恢复脂多糖转运蛋白的野生型功能，并表明 ATP 结合可触发转运。
mBio. 2019 Aug 20;10(4):e01931-19. doi: 10.1128/mBio.01931-19.
6
Lipopolysaccharide transport to the cell surface: biosynthesis and extraction from the inner membrane.脂多糖向细胞表面的转运：生物合成及从内膜的提取
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679). doi: 10.1098/rstb.2015.0029.
7
Lipopolysaccharide transport to the cell surface: periplasmic transport and assembly into the outer membrane.脂多糖转运至细胞表面：周质转运及在外膜中的组装
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679). doi: 10.1098/rstb.2015.0027.
8
Structural Basis for the Lipopolysaccharide Export Activity of the Bacterial Lipopolysaccharide Transport System.细菌脂多糖转运系统的脂多糖输出活性的结构基础。
Int J Mol Sci. 2018 Sep 10;19(9):2680. doi: 10.3390/ijms19092680.
9
Structural Insights into the Lipopolysaccharide Transport (Lpt) System as a Novel Antibiotic Target.脂多糖转运（Lpt）系统的结构见解作为一种新的抗生素靶标。
J Microbiol. 2024 Apr;62(4):261-275. doi: 10.1007/s12275-024-00137-w. Epub 2024 May 31.
10
Antimicrobial Agents That Inhibit the Outer Membrane Assembly Machines of Gram-Negative Bacteria.抑制革兰氏阴性菌外膜组装机器的抗菌剂。
J Microbiol Biotechnol. 2019 Jan 28;29(1):1-10. doi: 10.4014/jmb.1804.03051.

引用本文的文献

1
Antibiotic-resistant Acinetobacter baumannii can be killed by a combination of bacteriophages and complement.耐抗生素的鲍曼不动杆菌可被噬菌体和补体的组合杀死。
Med Microbiol Immunol. 2025 Sep 2;214(1):40. doi: 10.1007/s00430-025-00852-0.
2
Adaptive genetics reveals constraints on protein structure/function by evolving E. coli under constant nutrient limitation.适应性遗传学通过在恒定营养限制条件下培养大肠杆菌揭示了对蛋白质结构/功能的限制。
BMC Biol. 2025 Aug 20;23(1):261. doi: 10.1186/s12915-025-02331-7.
3
In silico analysis of zosurabalpin-LptB2FG binding in spp., , and : mechanisms underlying its differential efficacy.在硅基上对佐苏拉宾与嗜肺军团菌、肺炎军团菌和博兹曼军团菌中LptB2FG的结合进行分析：其差异疗效的潜在机制
In Silico Pharmacol. 2025 Apr 16;13(2):62. doi: 10.1007/s40203-025-00343-3. eCollection 2025.
4
LolA and LolB are conserved in Bacteroidota and are crucial for gliding motility and Type IX secretion.洛拉（LolA）和洛拉（LolB）在拟杆菌门中保守，对滑行运动和IX型分泌至关重要。
Commun Biol. 2025 Mar 6;8(1):376. doi: 10.1038/s42003-025-07817-2.
5
Immunogenic evaluation of LptD + LtgC as a bivalent vaccine candidate against Neisseria gonorrhoeae.LptD + LtgC作为抗淋病奈瑟菌二价疫苗候选物的免疫原性评估。
J Transl Med. 2025 Mar 4;23(1):261. doi: 10.1186/s12967-025-06256-1.
6
Identifying Opportunity Targets in Gram-Negative Pathogens for Infectious Disease Mitigation.确定革兰氏阴性病原体中用于减轻传染病的机会靶点。
ACS Cent Sci. 2025 Jan 3;11(1):25-35. doi: 10.1021/acscentsci.4c01437. eCollection 2025 Jan 22.
7
Single Disulfide Bond in Host Defense Thanatin Analog Peptides: Antimicrobial Activity, Atomic-Resolution Structures and Target Interactions.宿主防御肽那他菌素类似物中的单个二硫键：抗菌活性、原子分辨率结构及与靶点的相互作用
Int J Mol Sci. 2024 Dec 24;26(1):51. doi: 10.3390/ijms26010051.
8
Breaking Barriers: Exploiting Envelope Biogenesis and Stress Responses to Develop Novel Antimicrobial Strategies in Gram-Negative Bacteria.突破障碍：利用包膜生物发生和应激反应开发针对革兰氏阴性菌的新型抗菌策略
Pathogens. 2024 Oct 11;13(10):889. doi: 10.3390/pathogens13100889.
9
Thanatin: A Promising Antimicrobial Peptide Targeting the Achilles' Heel of Multidrug-Resistant Bacteria.Thanatin：一种有前途的靶向多重耐药菌阿喀琉斯之踵的抗菌肽。
Int J Mol Sci. 2024 Aug 31;25(17):9496. doi: 10.3390/ijms25179496.
10
Conformational investigation of the asymmetric periplasmic domains of LptBFGC using SDSL CW EPR spectroscopy.使用定点自旋标记连续波电子顺磁共振波谱法对LptBFGC的不对称周质结构域进行构象研究。
Appl Magn Reson. 2024 Mar;55(1-3):141-158. doi: 10.1007/s00723-023-01590-3. Epub 2023 Aug 7.

本文引用的文献

1
Interaction of lipopolysaccharides at intermolecular sites of the periplasmic Lpt transport assembly.脂多糖在周质 Lpt 转运组装体分子间部位的相互作用。
Sci Rep. 2017 Aug 29;7(1):9715. doi: 10.1038/s41598-017-10136-0.
2
Lipopolysaccharide binding to the periplasmic protein LptA.脂多糖与周质蛋白LptA结合。
Protein Sci. 2017 Aug;26(8):1517-1523. doi: 10.1002/pro.3177. Epub 2017 Apr 30.
3
Structural basis for lipopolysaccharide extraction by ABC transporter LptBFG.ABC 转运器 LptBFG 提取脂多糖的结构基础。
Nat Struct Mol Biol. 2017 May;24(5):469-474. doi: 10.1038/nsmb.3399. Epub 2017 Apr 10.
4
The β-barrel assembly machinery in motion.处于运转状态的β桶组装机制。
Nat Rev Microbiol. 2017 Apr;15(4):197-204. doi: 10.1038/nrmicro.2016.191. Epub 2017 Feb 20.
5
Bacterial lipoproteins; biogenesis, sorting and quality control.细菌脂蛋白；生物发生、分拣和质量控制。
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Nov;1862(11):1414-1423. doi: 10.1016/j.bbalip.2016.11.009. Epub 2016 Nov 19.
6
Identification of Residues in the Lipopolysaccharide ABC Transporter That Coordinate ATPase Activity with Extractor Function.鉴定脂多糖ABC转运蛋白中与ATP酶活性和提取功能相协调的残基。
mBio. 2016 Oct 18;7(5):e01729-16. doi: 10.1128/mBio.01729-16.
7
A penicillin-binding protein inhibits selection of colistin-resistant, lipooligosaccharide-deficient Acinetobacter baumannii.一种青霉素结合蛋白可抑制耐黏菌素、脂寡糖缺陷型鲍曼不动杆菌的选择。
Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6228-E6237. doi: 10.1073/pnas.1611594113. Epub 2016 Sep 28.
8
The Lack of the Essential LptC Protein in the Trans-Envelope Lipopolysaccharide Transport Machine Is Circumvented by Suppressor Mutations in LptF, an Inner Membrane Component of the Escherichia coli Transporter.大肠杆菌转运蛋白内膜组分LptF中的抑制突变规避了跨内膜脂多糖转运机器中必需的LptC蛋白的缺失。
PLoS One. 2016 Aug 16;11(8):e0161354. doi: 10.1371/journal.pone.0161354. eCollection 2016.
9
Mechanistic diversity in ATP-binding cassette (ABC) transporters.ATP 结合盒（ABC）转运蛋白的机制多样性。
Nat Struct Mol Biol. 2016 Jun 7;23(6):487-93. doi: 10.1038/nsmb.3216.
10
Functional Interaction between the Cytoplasmic ABC Protein LptB and the Inner Membrane LptC Protein, Components of the Lipopolysaccharide Transport Machinery in Escherichia coli.细胞质ABC蛋白LptB与内膜LptC蛋白之间的功能相互作用，二者为大肠杆菌脂多糖转运机制的组成成分。
J Bacteriol. 2016 Jul 28;198(16):2192-203. doi: 10.1128/JB.00329-16. Print 2016 Aug 15.