Department of neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Neurosci Bull. 2009 Aug;25(4):203-8. doi: 10.1007/s12264-009-0309-5.
To study the co-effect of procyanidins extracted from the lotus seed pod (LSPC) and bilobalide (BIL) on ameliorating scopolamine-induced learning and memory impairment in young mice.
Fifty male Kunming mice with similar learning and memory capabilities were selected by Morris water maze test and were randomized into 5 groups (n=10 in each group): control group, scopolamine group, L-(LSPC+BIL) group (50 mg/kg LSPC+10 mg/kg BIL), M-(LSPC+BIL) group (100 mg/kg LSPC+20 mg/kg BIL), H-(LSPC+BIL) group (150 mg/kg LSPC+30 mg/kg BIL). Scopolamine model with impaired learning and memory was established by scopolamine treatment (1 mg/kg), and after 10 min mice were tested. In L-, M-, and H-(LSPC+BIL) groups, mice were treated with LSPC and BIL ig. for 30 days, while mice in the other 2 groups were treated with normal saline ig. instead. After the 30-day's treatment, the co-effect of LSPC and BIL on learning and memory was tested by Morris water maze and the step-down avoidance tests.
The memory impairment caused by scopolamine in young mice could be ameliorated by co-treatment of LSPC and BIL, as indicated by significantly shorter escape latency and swimming distance in the Morris water maze test, when compared with those in the scopolamine group. In the step-down avoidance test, mice in all the 3 dose groups showed significantly smaller number of errors and longer latency than mice in the scopolamine group did.
Co-treatment of LSPE and BIL can ameliorate scopolamine-induced learning and memory impairment in young mice.
研究莲心低聚原花青素（LSPC）与银杏内酯 B（BIL）联合作用对改善年轻小鼠东莨菪碱诱导的学习记忆障碍的协同效应。
通过 Morris 水迷宫试验选择 50 只学习记忆能力相似的雄性昆明小鼠，随机分为 5 组（每组 10 只）：对照组、东莨菪碱组、低剂量（LSPC+BIL）组（50mg/kg LSPC+10mg/kg BIL）、中剂量（LSPC+BIL）组（100mg/kg LSPC+20mg/kg BIL）、高剂量（LSPC+BIL）组（150mg/kg LSPC+30mg/kg BIL）。东莨菪碱处理（1mg/kg）建立学习记忆损伤的东莨菪碱模型，处理 10min 后进行测试。在低、中、高剂量（LSPC+BIL）组中，小鼠 ig 给予 LSPC 和 BIL 治疗 30 天，而其他 2 组小鼠 ig 给予生理盐水代替。治疗 30 天后，通过 Morris 水迷宫和避错实验测试 LSPC 和 BIL 联合作用对学习记忆的影响。
与东莨菪碱组相比，LSPC 和 BIL 联合治疗可改善年轻小鼠东莨菪碱引起的记忆障碍，表现在 Morris 水迷宫试验中逃避潜伏期和游泳距离明显缩短。在避错实验中，所有 3 个剂量组的小鼠错误次数明显少于东莨菪碱组，潜伏期明显长于东莨菪碱组。
LSPE 和 BIL 联合治疗可改善年轻小鼠东莨菪碱诱导的学习记忆障碍。
