17β-雌二醇调节的突触可塑性对阿尔茨海默病大鼠学习记忆的影响。
Effects of synaptic plasticity regulated by 17beta-estradiol on learning and memory in rats with Alzheimer's disease.
机构信息
Department of Neurology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
出版信息
Neurosci Bull. 2010 Apr;26(2):133-9. doi: 10.1007/s12264-010-0913-4.
OBJECTIVE
To investigate whether estrogen modulates learning and memory and long-term potentiation (LTP) in the hippocampus of rats with Alzheimer's disease (AD).
METHODS
The rats were divided into ovariectomy (OVX) and estrogen replacement therapy (ERT) groups. Rats in the ERT group received OVX, followed by ERT, while rats in the OVX group received only OVX. The rat model of AD was established by injection of 1 microL (10 microg/microL) amyloid-beta peptide 1-40(Abeta1-40) into the hippocampus. The learning and memory ability and LTP were determined by Morris water maze and electrophysiological method, respectively.
RESULTS
The escape latency in Morris water maze significantly decreased in ERT group compared with that in OVX group (P< 0.05). Besides, rats in ERT group exhibited a significant enhancement of the magnitude of LTP at 30 min after high-frequency stimulation (HFS), compared with that in OVX group (P< 0.01).
CONCLUSION
ERT can attenuate the cognitive deficits in the rat model of AD, and estrogen can regulate LTP and synaptic remodeling in AD rats.
目的
研究雌激素是否能调节阿尔茨海默病（AD）大鼠海马的学习记忆和长时程增强（LTP）。
方法
将大鼠分为卵巢切除术（OVX）和雌激素替代治疗（ERT）组。ERT 组大鼠接受 OVX 后进行 ERT，而 OVX 组大鼠仅接受 OVX。通过海马内注射 1 微升（10 微克/微升）淀粉样蛋白-β肽 1-40（Abeta1-40）建立 AD 大鼠模型。通过 Morris 水迷宫和电生理方法分别测定学习记忆能力和 LTP。
结果
ERT 组大鼠在 Morris 水迷宫中的逃避潜伏期明显短于 OVX 组（P<0.05）。此外，ERT 组大鼠在高频刺激（HFS）后 30 分钟时 LTP 的幅度明显增强，与 OVX 组相比差异有统计学意义（P<0.01）。
结论
ERT 可减轻 AD 大鼠的认知功能障碍，雌激素可调节 AD 大鼠的 LTP 和突触重塑。
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