当多种耐甲氧西林金黄色葡萄球菌（MRSA）菌株引发流行时，对一种商用MRSA检测方法的评估。
Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics.
作者信息
Sissonen S, Pasanen T, Salmenlinna S, Vuopio-Varkila J, Tarkka E, Vaara M, Tissari P
机构信息
Department of Bacteriology, Division of Clinical Microbiology, HUSLAB, Helsinki University Hospital, P.O. Box 400 (Haartmaninkatu 3), 00029 HUS, Helsinki, Finland.
出版信息
Eur J Clin Microbiol Infect Dis. 2009 Oct;28(10):1271-3. doi: 10.1007/s10096-009-0771-z. Epub 2009 Jul 25.
Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.
为控制耐甲氧西林金黄色葡萄球菌（MRSA）的传播，需要快速且可靠的诊断方法。我们研究了BD GeneOhm MRSA检测方法，该方法基于葡萄球菌盒式染色体mec（SCCmec）右端序列与金黄色葡萄球菌orfX染色体序列交界处的一个特定扩增产物。该检测方法应用于芬兰的95株临床分离株：83%为阳性。检测结果为阴性的分离株代表几种脉冲场凝胶电泳（PFGE）类型，携带IV型、V型、VI型SCCmec，或为具有不同ccr基因组合的新型别。
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