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基于快速聚合酶链反应从筛查拭子中鉴定耐甲氧西林金黄色葡萄球菌

Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs.

作者信息

Jonas D, Speck M, Daschner F D, Grundmann H

机构信息

National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany.

出版信息

J Clin Microbiol. 2002 May;40(5):1821-3. doi: 10.1128/JCM.40.5.1821-1823.2002.

DOI:10.1128/JCM.40.5.1821-1823.2002
PMID:11980967
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC130653/
Abstract

A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.

摘要

研究了一种用于耐甲氧西林金黄色葡萄球菌（MRSA）的PCR鉴定方法，该方法无需在琼脂培养基上进行传代培养。通过mecA femB PCR检测MRSA并结合在选择性肉汤中预先富集的方法，对439份拭子样本进行了测试。PCR鉴定出36份MRSA阳性样本，与传统方法结果一致。

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