Department of Medicine, Turku University Hospital and University of Turku, Kiinamyllynkatu 4-8, 20520 Turku, Finland.
Eur J Clin Microbiol Infect Dis. 2011 Oct;30(10):1237-43. doi: 10.1007/s10096-011-1218-x. Epub 2011 Apr 2.
The aim of this study was to evaluate a new type of assay for the phenotypic detection of methicillin-resistant Staphylococcus aureus (MRSA). The assay is based on a point-of-care compatible two-photon excitation fluorescence detection technology (TPX). A collection of 243 epidemic MRSA isolates was tested in addition to 138 sporadic MRSA and 101 negative control strains. The assay proved to be both sensitive (97.9%) and specific (94.1%) in the identification of MRSA, with adequate positive (98.4%) and negative (92.2%) predictive values. The time required for obtaining a positive test result was less than 14 h for 99.0% of the MRSA true-positive samples. After a test run, the selectively enriched reaction mixtures may be recovered and further studied by molecular or standard phenotypic methods. The main benefits of the TPX methodology include a simple assay procedure, low reagent consumption, and a high-throughput capacity.
本研究旨在评估一种新型的用于检测耐甲氧西林金黄色葡萄球菌（MRSA）表型的检测方法。该方法基于一种即时检测兼容的双光子激发荧光检测技术（TPX）。除了 138 株散发性 MRSA 和 101 株阴性对照菌株外，我们还对 243 株流行的 MRSA 分离株进行了检测。该方法在鉴定 MRSA 方面具有较高的敏感性（97.9%）和特异性（94.1%），阳性预测值（98.4%）和阴性预测值（92.2%）均较为充足。对于 99.0%的 MRSA 真阳性样本，获得阳性检测结果所需的时间少于 14 小时。测试完成后，可以回收选择性富集的反应混合物，并通过分子或标准表型方法进一步研究。TPX 方法学的主要优点包括简单的检测程序、低试剂消耗和高通量能力。
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