Rehman Muhammad Attiq, Yankulov Krassimir
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Curr Genet. 2009 Aug;55(4):357-63. doi: 10.1007/s00294-009-0265-7. Epub 2009 Jul 26.
Autonomously replicating sequences (ARSs) in Saccharomyces cerevisiae have been extensively characterized as both origins of DNA replication and as chromatin repressors/silencers. It has been conclusively shown that the origin and the silencer activities of ARS are substantially, but not entirely interchangeable and that they are modulated by position effects and chromatin environment. It remains unclear how these two quite divergent functions of ARS co-exist. This perspective focuses on recent advances, which have shown that slight differences in ARSs can modulate their affinity for origin recognition complex and their activity as silencers or origins.
酿酒酵母中的自主复制序列（ARSs）已被广泛表征为DNA复制起点以及染色质阻遏物/沉默子。已有确凿证据表明，ARS的起点和沉默子活性在很大程度上但并非完全可互换，并且它们受到位置效应和染色质环境的调节。目前尚不清楚ARS的这两种截然不同的功能是如何共存的。本文观点聚焦于最近的进展，这些进展表明ARSs的细微差异可调节它们对起点识别复合物的亲和力以及作为沉默子或起点的活性。
