Kearsey S
Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge, UK.
EMBO J. 1983;2(9):1571-5. doi: 10.1002/j.1460-2075.1983.tb01626.x.
A method is presented for rapid sequencing and mapping of elements which support autonomous replication in yeast. The strategy relies on a novel phage M13 vector which allows detection of ARS (autonomously replicating sequence) function in cloned fragments. Deletion mapping of an ARS element linked to the HO gene of Saccharomyces cerevisiae has identified a 57-bp region 3' to the gene, which is essential for autonomous replication. This region shows sequence homology to other ARS elements.
本文介绍了一种用于快速测序和定位支持酵母自主复制元件的方法。该策略依赖于一种新型噬菌体M13载体,它能够检测克隆片段中的ARS(自主复制序列)功能。对与酿酒酵母HO基因相连的ARS元件进行缺失定位,已确定该基因3'端有一个57 bp的区域,它对自主复制至关重要。该区域与其他ARS元件具有序列同源性。