Satou Tadaaki, Matsuura Mariko, Murakami Shio, Hayashi Shinichiro, Koike Kazuo
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Nat Prod Commun. 2009 Jun;4(6):845-8.
The composition of the steam-distilled essential oil from the leaves of Abies sachalinensis (F. Schmidt) Mast. cultivated in Hokkaido (Japan) was studied by GC-MS. The seasonal variation in the main volatile constituents was also investigated. Analysis of the essential oil resulted in the identification of 21 compounds with monoterpenes comprising 99.9% of the total. alpha-Pinene was the most abundant compound, followed by camphene, bornyl acetate, limonene, beta-pinene and beta-phellandrene. The sesquiterpene content was low, and was mainly represented by beta-caryophyllene, beta-caryophyllene, and gamma-selinene. The essential oil from the leaves, collected at eight different collection periods over more than 5 years, showed significant differences in composition. Alpha-Pinene was the predominant constituent during the collection periods, with a few exceptions. The alpha-pinene content of the oil was abundant in April-June, and decreased in November-December. Levels of bornyl acetate showed the greatest increase in December, when the temperature was very low. The enantiomeric distribution of alpha-pinene was suggested to relate to seasonal transformation. (+)-alpha-Pinene showed a tendency to increase when total precipitation, average temperature, and total duration of sunshine were high.
对日本北海道种植的库页冷杉（Abies sachalinensis (F. Schmidt) Mast.）叶片水蒸气蒸馏所得精油的成分进行了气相色谱 - 质谱联用（GC - MS）分析研究。同时也对主要挥发性成分的季节变化进行了调查。精油分析鉴定出21种化合物，其中单萜类化合物占总量的99.9%。α - 蒎烯是含量最丰富的化合物，其次是莰烯、乙酸龙脑酯、柠檬烯、β - 蒎烯和β - 水芹烯。倍半萜含量较低，主要为β - 石竹烯、β - 石竹烯和γ - 芹子烯。在5年多时间里于8个不同采集期采集的叶片精油，其成分存在显著差异。除少数例外情况，α - 蒎烯在各采集期都是主要成分。精油中α - 蒎烯含量在4 - 6月丰富，11 - 12月减少。乙酸龙脑酯含量在12月温度很低时增长幅度最大。α - 蒎烯的对映体分布被认为与季节变化有关。当总降水量、平均温度和日照总时长较高时，( + ) - α - 蒎烯呈增加趋势。
