Sun Xiao-hong, Meng Wei-shan, Fu Shi-hong, Feng Yun, Zhai You-gang, Wang Jing-lin, Wang Huan-qin, Lv Xin-jun, Liang Guo-dong
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Bing Du Xue Bao. 2009 May;25(3):173-7.
5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain JKT-7075 in RT-PCR. The full length of the 12th segment was 758 nucleotides, BLAST analysis revealed the highest identity was 90% to JKT-7075. Phylogenetic analysis demonstrated that the isolates appeared to be Kadipiro viruses (Family Reoviridae). It was the first report of kadipiro virus isolation in China.
在中国云南省西北部虫媒病毒调查中，已从三带喙库蚊、中华按蚊和白纹伊蚊中分离出5株病毒，这些病毒在C6/36细胞中引起细胞病变效应。病毒粒子直径为70纳米（n = 7），无包膜但表面有刺突。分离株基因组的RNA-PAGE显示为6-5-1图谱。通过一对针对卡迪皮罗病毒株JKT-7075的特异性引物在RT-PCR中扩增出第12节段序列的一个片段。第12节段全长758个核苷酸，BLAST分析显示与JKT-7075的最高同源性为90%。系统发育分析表明，分离株似乎是呼肠孤病毒科的卡迪皮罗病毒。这是中国首次报道分离出卡迪皮罗病毒。
