中国山东省分离的 Kadipiro 病毒的鉴定和遗传分析。
Identification and genetic analysis of Kadipiro virus isolated in Shandong province, China.
机构信息
School of Public Health, Shandong University, Jinan, 250012, People's Republic of China.
State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
出版信息
Virol J. 2018 Apr 6;15(1):64. doi: 10.1186/s12985-018-0966-y.
BACKGROUND
Kadipiro virus (KDV) belongs to the Reoviridae family, which consists of segmented, non-enveloped, double-stranded RNA viruses. It has previously been isolated from Culex, Anopheles, Armigeres and Aedes mosquitoes in Indonesia and China. Here, we describe the isolation and characterization of SDKL1625 from Anopheles sinensis mosquitoes in Shandong province, China.
METHODS
In this study, we isolated Kadipiro virus in Aedes albopictus C6/36 cell culture and the complete genome sequencing was made by next generation sequencing.
RESULTS
We isolated and characterized a Kadipiro virus from Anopheles sinensis mosquitoes in 2016 in Shandong province, China. Nucleotide and amino acid homology analysis of SDKL1625 showed higher levels of sequence identity with QTM27331 (Odonata, China, 2016) than with JKT-7075 (Culex fuscocephalus, Indonesia, 1981). The SDKL1625 has 86-97% amino acid identity with the JKT-7075, 88-99% amino acid identity with the QTM27331. Among the 12 fragments, VP1, VP2, VP4, VP6, VP7, VP9 and VP12 showed high amino acid identity (> 90%) and VP5 showed the lowest identity (86% and 88%).
CONCLUSIONS
This is the first identification of KDV from mosquito in China. Virus morphology and genome organization were also determined, which will further enrich our understanding of the molecular biological characteristics of KDV and seadornaviruses.
背景
卡迪波病毒（KDV）属于呼肠孤病毒科，该科由分段、无包膜、双链 RNA 病毒组成。它之前已从印度尼西亚和中国的库蚊、按蚊、伊蚊属和库蚊属蚊虫中分离出来。在这里，我们描述了从中国山东省中华按蚊中分离的 SDKL1625 并对其进行了特征描述。
方法
在本研究中，我们在白纹伊蚊 C6/36 细胞培养物中分离出卡迪波病毒，并通过下一代测序完成了全基因组测序。
结果
我们从中国山东省 2016 年的中华按蚊中分离并鉴定出一种卡迪波病毒。SDKL1625 的核苷酸和氨基酸同源性分析显示，与 QTM27331（中国，2016 年，蜻蜓目）相比，与 JKT-7075（印度尼西亚，1981 年，库蚊属）的序列同一性更高。SDKL1625 与 JKT-7075 的氨基酸同一性为 86-97%，与 QTM27331 的氨基酸同一性为 88-99%。在这 12 个片段中，VP1、VP2、VP4、VP6、VP7、VP9 和 VP12 显示出高氨基酸同一性（>90%），而 VP5 显示出最低的同一性（86%和 88%）。
结论
这是在中国首次从蚊虫中鉴定出 KDV。还确定了病毒形态和基因组组织，这将进一步丰富我们对 KDV 和海德尔堡病毒分子生物学特征的认识。
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