Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Neural Regen Res. 2012 Dec 5;7(34):2689-97. doi: 10.3969/j.issn.1673-5374.2012.34.006.
This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or Aβ1-40-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells.
本研究旨在探讨在损伤神经细胞诱导下，人脐带间充质干细胞（hUCMSCs）的神经分化。体外分离培养后，选用第 5 代 hUCMSCs 进行实验。将 hUCMSCs 与正常或 Aβ1-40 损伤的 PC12 细胞、PC12 细胞上清液或 PC12 细胞裂解物在 Transwell 共培养系统中共培养。Western blot 分析和流式细胞术结果显示，在各种培养条件下，胆碱乙酰转移酶和微管相关蛋白 2（神经细胞的特异性标志物）在 hUCMSCs 中表达，与损伤的 PC12 细胞共培养的 hUCMSCs 表达最高。单独培养的 hUCMSCs（无处理）中不表达胆碱乙酰转移酶和微管相关蛋白 2。细胞计数试剂盒-8 检测结果表明，共培养条件下的 hUCMSCs 可促进损伤的 PC12 细胞增殖。这些发现表明，神经组织损伤期间的微环境可有效诱导 hUCMSCs 的神经细胞分化。这些分化的 hUCMSCs 可能加速损伤神经细胞的修复。
