Janah S, Mali B, Fleck A
Department of Chemical Pathology, Charing Cross and Westminster Medical School, London, U.K.
FEMS Microbiol Immunol. 1990 Dec;2(5-6):313-9. doi: 10.1111/j.1574-6968.1990.tb03534.x.
The effect of the external cations Na+ and Ca2+ on polymorphonuclear chemiluminescence was investigated. Both Ca2+ in the range of 0.2-2 mM and Na+ in the range of 114-143 mM showed a dose dependent increase in polymorphonuclear chemiluminescence, irrespective of the concurrent increase in osmolality. The Na+/H+ antiport inhibitor Amiloride decreased the response significantly. These effects were observed using buffers commonly used for chemiluminescence studies and indicate the importance of defining the Ca2+ and Na+ composition of the buffers used in chemiluminescence assays.
研究了外部阳离子Na+和Ca2+对多形核白细胞化学发光的影响。0.2 - 2 mM范围内的Ca2+和114 - 143 mM范围内的Na+均显示多形核白细胞化学发光呈剂量依赖性增加，与渗透压的同时增加无关。Na+/H+反向转运抑制剂阿米洛利显著降低了该反应。使用化学发光研究常用的缓冲液观察到了这些效应，这表明确定化学发光测定中所用缓冲液的Ca2+和Na+组成很重要。
