一种用于快速鉴定光滑念珠菌及其系统发育相关物种尼瓦念珠菌和布拉卡念珠菌的多重PCR方案。
A multiplex PCR protocol for rapid identification of Candida glabrata and its phylogenetically related species Candida nivariensis and Candida bracarensis.
作者信息
Romeo Orazio, Scordino Fabio, Pernice Ida, Lo Passo Carla, Criseo Giuseppe
机构信息
Department of Life Sciences "Marcello Malpighi", Section of Microbiological, Genetic and Molecular Sciences, University of Messina, Salita Sperone 31, 98166 Messina, Italy.
出版信息
J Microbiol Methods. 2009 Oct;79(1):117-20. doi: 10.1016/j.mimet.2009.07.016. Epub 2009 Jul 25.
We have developed a multiplex PCR protocol for the detection of Candida glabrata and its closely related species Candida nivariensis and Candida bracarensis. The method uses four PCR primers, targeting the ITS1 region and the 5.8S ribosomal RNA gene. The combination of these primers yielded unique results to all Candida species tested. The PCR assay we developed was found to be a rapid, specific and easy to perform method and it will be useful for characterizing large numbers of isolates for epidemiological studies.
我们开发了一种多重PCR方法，用于检测光滑念珠菌及其密切相关的物种—— nivariensis念珠菌和布拉卡念珠菌。该方法使用四种PCR引物，靶向ITS1区域和5.8S核糖体RNA基因。这些引物的组合对所有测试的念珠菌物种都产生了独特的结果。我们开发的PCR检测方法被发现是一种快速、特异且易于操作的方法，它将有助于对大量分离株进行表征，以用于流行病学研究。
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