基于上转换磷光技术的侧向流动分析法快速定量检测布鲁氏菌
Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay.
作者信息
Qu Qing, Zhu Ziwen, Wang Yufei, Zhong Zhijun, Zhao Jin, Qiao Feng, Du Xinying, Wang Zhoujia, Yang Ruifu, Huang Liuyu, Yu Yaqin, Zhou Lei, Chen Zeliang
机构信息
Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing 100071, China.
出版信息
J Microbiol Methods. 2009 Oct;79(1):121-3. doi: 10.1016/j.mimet.2009.07.015. Epub 2009 Jul 25.
A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.
为实现布鲁氏菌的现场检测，开发了一种基于快速定量上转换磷光体技术的侧向流动分析方法（UPT-LF分析）。无论是纯培养物还是加标样品中的不同布鲁氏菌物种都可以进行定量检测。纯培养物的检测限为5×10⁶CFU/ml，不同加标样品的灵敏度范围为2.0×10³至3.9×10⁵CFU/mg。UPT-LF分析具有高特异性、重现性和稳定性，为布鲁氏菌的现场检测提供了巨大潜力。
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