INSERM U629, F-59019 Lille, France.
Microbes Infect. 2009 Nov;11(13):995-1001. doi: 10.1016/j.micinf.2009.07.005. Epub 2009 Jul 25.
Pulmonary tuberculosis remains a major health problem. Effective vaccination strategies are urgently needed. It was previously demonstrated that purified Mycobacterium bovis BCG Heparin Binding Haemagglutinin (HBHA) is able to induce in BALB/c mice protection levels against a Mycobacterium tuberculosis infection that are similar to those offered by BCG. Here we developed a heterologous prime/boost immunisation approach using a combination of BCG and HBHA in order to increase the protective immune response. We show that the time period between BCG priming and HBHA boosting strongly influences the efficacy of the boost. The optimized vaccine protocol consisting of a BCG administration followed 8 months later by boosting with HBHA resulted in an increase in the level of protection of up to 0.7log when compared to BCG alone. These results suggest an immunisation strategy where BCG is administered to neonates and is followed by subcutaneous HBHA boosting in young adults.
肺结核仍然是一个主要的健康问题。迫切需要有效的疫苗接种策略。此前已经证明，纯化的牛结核分枝杆菌肝素结合血凝素（HBHA）能够诱导 BALB/c 小鼠对结核分枝杆菌感染的保护水平与卡介苗相当。在这里，我们使用卡介苗和 HBHA 的组合开发了一种异源初免/加强免疫接种方法，以增强保护性免疫反应。我们表明，卡介苗初免和 HBHA 加强之间的时间间隔强烈影响加强的效果。与单独使用卡介苗相比，由卡介苗接种后 8 个月再用 HBHA 加强组成的优化疫苗方案可使保护水平提高高达 0.7log。这些结果表明了一种免疫接种策略，即在新生儿中接种卡介苗，然后在年轻人中皮下注射 HBHA 加强。
