Jansson Janet, Willing Ben, Lucio Marianna, Fekete Ages, Dicksved Johan, Halfvarson Jonas, Tysk Curt, Schmitt-Kopplin Philippe
Ecology Department, Division of Earth Sciences, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America.
PLoS One. 2009 Jul 28;4(7):e6386. doi: 10.1371/journal.pone.0006386.
The causes and etiology of Crohn's disease (CD) are currently unknown although both host genetics and environmental factors play a role. Here we used non-targeted metabolic profiling to determine the contribution of metabolites produced by the gut microbiota towards disease status of the host. Ion Cyclotron Resonance Fourier Transform Mass Spectrometry (ICR-FT/MS) was used to discern the masses of thousands of metabolites in fecal samples collected from 17 identical twin pairs, including healthy individuals and those with CD. Pathways with differentiating metabolites included those involved in the metabolism and or synthesis of amino acids, fatty acids, bile acids and arachidonic acid. Several metabolites were positively or negatively correlated to the disease phenotype and to specific microbes previously characterized in the same samples. Our data reveal novel differentiating metabolites for CD that may provide diagnostic biomarkers and/or monitoring tools as well as insight into potential targets for disease therapy and prevention.
尽管宿主遗传学和环境因素都在克罗恩病（CD）中发挥作用，但其病因和发病机制目前仍不清楚。在此，我们采用非靶向代谢组学分析，以确定肠道微生物群产生的代谢产物对宿主疾病状态的影响。利用离子回旋共振傅里叶变换质谱（ICR-FT/MS）分析了17对同卵双胞胎（包括健康个体和CD患者）粪便样本中数千种代谢产物的质量。具有差异代谢产物的途径包括那些参与氨基酸、脂肪酸、胆汁酸和花生四烯酸代谢和/或合成的途径。几种代谢产物与疾病表型以及先前在相同样本中鉴定的特定微生物呈正相关或负相关。我们的数据揭示了CD新的差异代谢产物，这些代谢产物可能提供诊断生物标志物和/或监测工具，并有助于深入了解疾病治疗和预防的潜在靶点。
