Francois-Pierre Martin, Seu Ping Guiraud, Martin Kussmann, Nestlé Institute of Health Sciences, 1015 Lausanne, Switzerland.
World J Gastroenterol. 2017 May 28;23(20):3643-3654. doi: 10.3748/wjg.v23.i20.3643.
To identify metabolic signatures in urine samples from healthy and inflammatory bowel disease (IBD) children.
We applied liquid chromatography and gas chromatography coupled to targeted mass spectrometry (MS)-based metabolite profiling to identify and quantify bile acids and host-gut microbial metabolites in urine samples collected from 21 pediatric IBD patients monitored three times over one year (baseline, 6 and 12 mo), and 27 age- and gender-matched healthy children.
urinary metabolic profiles of IBD children differ significantly from healthy controls. Such metabolic differences encompass central energy metabolism, amino acids, bile acids and gut microbial metabolites. In particular, levels of pyroglutamic acid, glutamic acid, glycine and cysteine, were significantly higher in IBD children in the course of the study. This suggests that glutathione cannot be optimally synthesized and replenished. Whilst alterations of the enterohepatic circulation of bile acids in pediatric IBD patients is known, we show here that non-invasive urinary bile acid profiling can assess those altered hepatic and intestinal barrier dysfunctions.
The present study shows how non-invasive sampling of urine followed by targeted MS-based metabonomic analysis can elucidate and monitor the metabolic status of children with different GI health/disease status.
鉴定健康和炎症性肠病（IBD）儿童尿液样本中的代谢特征。
我们应用液相色谱和气相色谱与靶向质谱（MS）联用的代谢组学方法，鉴定和定量来自 21 名接受为期一年（基线、6 个月和 12 个月）三次监测的儿科 IBD 患者和 27 名年龄和性别匹配的健康儿童的尿液样本中的胆汁酸和宿主-肠道微生物代谢物。
IBD 儿童的尿液代谢谱与健康对照组有显著差异。这些代谢差异包括中枢能量代谢、氨基酸、胆汁酸和肠道微生物代谢物。特别是，在研究过程中，IBD 儿童的焦谷氨酸、谷氨酸、甘氨酸和半胱氨酸水平明显升高。这表明谷胱甘肽不能被最佳合成和补充。虽然已经知道儿科 IBD 患者的胆汁酸肠肝循环发生改变，但我们在这里表明，非侵入性尿液胆汁酸分析可以评估那些改变的肝和肠道屏障功能障碍。
本研究表明，通过非侵入性的尿液采样，然后进行靶向 MS 代谢组学分析，可以阐明和监测具有不同胃肠道健康/疾病状态的儿童的代谢状态。
