Kim Unyoung, Soh H Tom
Department of Mechanical Engineering, University of California, Santa Barbara, USA.
Lab Chip. 2009 Aug 21;9(16):2313-8. doi: 10.1039/b903950c. Epub 2009 May 14.
The ability to rapidly and accurately sort multiple types of biological targets-such as molecules, viruses, bacteria or mammalian cells-from complex sample mixtures is an essential component for a wide range of diagnostic and therapeutic strategies. However, most current selection methods for cell separation are either limited with regard to throughput, as is the case for Fluorescence Assisted Cell Sorting (FACS), or else only allow binary separation of targets that have been labeled via a single parameter, such as Magnetic Activated Cell Sorting (MACS). We report here the integrated Dielectrophoretic-Magnetic Activated Cell Sorter (iDMACS), an integrated platform that combines two different force fields in a single microfluidic device for highly efficient multi-target separation. We describe the underlying physics and design of the iDMACS device and demonstrate approximately 900-fold enrichment of multiple bacterial target cell types with over 95% purity after a single round of separation.
能够从复杂的样品混合物中快速、准确地分选多种类型的生物靶标，如分子、病毒、细菌或哺乳动物细胞，是广泛的诊断和治疗策略的重要组成部分。然而，目前大多数用于细胞分离的选择方法要么在通量方面受到限制，如荧光辅助细胞分选（FACS）的情况，要么只允许对通过单个参数标记的靶标进行二元分离，如磁激活细胞分选（MACS）。我们在此报告集成介电泳 - 磁激活细胞分选仪（iDMACS），这是一个在单个微流控装置中结合两种不同力场以实现高效多靶标分离的集成平台。我们描述了iDMACS装置的基础物理原理和设计，并展示了在一轮分离后，多种细菌靶细胞类型的富集倍数约为900倍，纯度超过95%。
