用于定量蛋白质组学的标准肽段的准确定量。
Accurate quantitation of standard peptides used for quantitative proteomics.
作者信息
Bordeerat Narisa K, Georgieva Nadia I, Klapper David G, Collins Leonard B, Cross Tyra J, Borchers Christoph H, Swenberg James A, Boysen Gunnar
机构信息
Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, USA.
出版信息
Proteomics. 2009 Aug;9(15):3939-44. doi: 10.1002/pmic.200900043.
Abstract
MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.
摘要
基于质谱的蛋白质组学已成为系统生物学中不可或缺的工具，这就需要对肽标准品进行准确且精确的定量。本文所提出的方法利用超高效液相色谱-串联质谱（UPLC-MS/MS）对浓度为0.1-10微摩尔的肽标准品进行准确定量。对微摩尔浓度进行准确定量的能力具有以下优势：定量可常规高精度地进行，且该方法的高灵敏度使所需量最小化。
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