乳胶凝集试验和斑点免疫结合试验检测巨细胞病毒抗体的评估

Evaluation of latex agglutination and dot immunobinding assay for the detection of cytomegalovirus antibody.

作者信息

Wilson M T, Sautter R L, LeBar W D, Mervak T R

机构信息

Microbiology Laboratory, Harrisburg Hospital, Pennsylvania.

出版信息

Diagn Microbiol Infect Dis. 1990 Jul-Aug;13(4):303-6. doi: 10.1016/0732-8893(90)90021-m.

DOI:10.1016/0732-8893(90)90021-m

PMID:1963835

Abstract

The Virogen CMV Antibody Test and Difco CMV-Cube were compared with three other available methods, enzyme immunoassay, immunofluorescence and latex agglutination, for the detection of cytomegalovirus (CMV) antibody in 126 random sera submitted to the clinical laboratory. Based on concordance of three or more methods, 72% of the samples were positive for CMV antibody. The sensitivities and specificities of the assays were Virogen, 98% and 97%, and CMV-Cube 97% and 97%, respectively.

摘要

将维洛金巨细胞病毒抗体检测法和迪夫科巨细胞病毒检测试剂盒与其他三种可用方法（酶免疫测定法、免疫荧光法和乳胶凝集法）进行比较，以检测提交至临床实验室的126份随机血清中的巨细胞病毒（CMV）抗体。基于三种或更多方法的一致性，72%的样本CMV抗体呈阳性。这些检测方法的灵敏度和特异性分别为：维洛金检测法，98%和97%；巨细胞病毒检测试剂盒，97%和97%。