Sodkiewicz W, Strzembicka A, Sodkiewicz T, Majewska M
Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.
J Appl Genet. 2009;50(3):205-11. doi: 10.1007/BF03195674.
Triticale introgressive lines were developed by incorporating diploid wheat (Triticum monococcum [TM16]) genes into the hexaploid triticale genotype LT522/6. The synthetic allotetraploid T. monococcum cereale (A(m) A(m)RR) was used as a bridging form to introduce the genes. A group of 43 introgressive lines, parental stocks and a check cultivar were inoculated at the seedling stage (in the greenhouse) and at the adult plant stage (in the field) with four pathotypes of Puccinia striiformis f. sp. tritici to determine if the stripe rust resistance was derived from TM16 and to analyze the expression of the diploid wheat gene(s) at the hexaploid level. At the seedling stage, 14 triticale introgressive lines expressed resistance to some of the used pathotypes, showing introduction of a genetic material from the T. monococcum genome. Among them, 7 lines were resistant to all four stripe rust pathotypes applied at this stage. In the field, adult plant resistance and percentage of infected leaf area were scored and transformed into the coefficient of infection. Plant response to stripe rust was compatible at these two developmental stages with a high statistical probability showing the genetic dependence on the same genetic background. Also observed was a full concordance of the adult plant resistance to stripe rust with previously assessed resistance to leaf rust, as well as the highly significant linkage of the resistance to the both diseases at the seedling stage in the set of the tested introgression lines. This result strongly suggests that T. monococcum genes responsible for these characters are located in proximity.
通过将二倍体小麦（一粒小麦[TM16]）基因导入六倍体小黑麦基因型LT522/6，培育出了小黑麦渐渗系。合成异源四倍体一粒小麦（A(m)A(m)RR）被用作桥梁形式来导入这些基因。在幼苗期（温室中）和成株期（田间），用小麦条锈菌的四个致病型对一组43个渐渗系、亲本材料和一个对照品种进行接种，以确定条锈病抗性是否源自TM16，并分析二倍体小麦基因在六倍体水平上的表达情况。在幼苗期，14个小黑麦渐渗系对部分所用致病型表现出抗性，表明从一粒小麦基因组中导入了遗传物质。其中，7个品系对该阶段所用的所有四种条锈病致病型均有抗性。在田间，对成株抗性和感染叶面积百分比进行评分并转化为感染系数。在这两个发育阶段，植株对条锈病的反应具有高度统计学概率的一致性，表明其遗传依赖于相同的遗传背景。还观察到成株对条锈病的抗性与先前评估的对叶锈病的抗性完全一致，以及在测试的渐渗系群体中，幼苗期对这两种病害的抗性存在高度显著的连锁关系。这一结果强烈表明，负责这些性状的一粒小麦基因位于相近位置。
