Tolmasky M E
Department of Microbiology and Immunology, School of Medicine, Oregon Health Sciences University, Portland 97201-3098.
Plasmid. 1990 Nov;24(3):218-26. doi: 10.1016/0147-619x(90)90005-w.
A fragment of Tn1331 including tnpR, aac, aadA, and a bla gene which encodes lower levels of resistance to ampicillin and carbenicillin as compared to those mediated by the TEM beta-lactamase was sequenced. The polypeptide encoded by the bla gene has homology with the OXA-1, PSE-2, and OXA-2 proteins. Genes aac and bla are upstream and downstream respectively of aadA, and are both flanked by recombinational hot spots. Tn1331 has 520-bp direct repeats which include parts of the tnpR and TEM bla genes. Evolutionary models for the genesis of Tn1331 are proposed.
对包含tnpR、aac、aadA和bla基因的Tn1331片段进行了测序，与TEMβ-内酰胺酶介导的相比，该bla基因编码的对氨苄青霉素和羧苄青霉素的耐药水平较低。bla基因编码的多肽与OXA-1、PSE-2和OXA-2蛋白具有同源性。基因aac和bla分别位于aadA的上游和下游，且两者均侧翼有重组热点。Tn1331有520 bp的正向重复序列，其中包括tnpR和TEM bla基因的部分序列。提出了Tn1331起源的进化模型。
