Arakawa Y, Ohta M, Kido N, Fujii Y, Komatsu T, Kato N
FEBS Lett. 1986 Oct 20;207(1):69-74. doi: 10.1016/0014-5793(86)80014-x.
Sixty-three percent homology of nucleotide sequence and 67% homology of deduced amino acid sequence were found between the chromosomally encoded beta-lactamase gene of Klebsiella pneumoniae and the TEM beta-lactamase of transposon Tn3. Moreover, 22 out of 24 amino acid residues are identical around the predicted active site. It is therefore suggested that these two kinds of beta-lactamases share a common evolutionary origin. The 0.5 kb DNA fragment of the cloned gene hybridized specifically with the chromosomal DNA of all the K. pneumoniae strains tested which had been isolated in Japan, USA and Europe.
肺炎克雷伯菌染色体编码的β-内酰胺酶基因与转座子Tn3的TEMβ-内酰胺酶之间，核苷酸序列同源性为63%，推导的氨基酸序列同源性为67%。此外，在预测的活性位点周围，24个氨基酸残基中有22个是相同的。因此，提示这两种β-内酰胺酶有着共同的进化起源。克隆基因的0.5kb DNA片段与在日本、美国和欧洲分离的所有受试肺炎克雷伯菌菌株的染色体DNA发生特异性杂交。
