Shi J, Fan Y, Xu R, Zuo K, Cheng L, Xu G, Li H
Allergy and Cancer Center, Otorhinolarygology Hospital of The First Affiliated Hospital of Sun Yat-sen University, and Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, China.
J Investig Allergol Clin Immunol. 2009;19(4):276-82.
Nasal polyposis has different etiologies in Western and Eastern countries. Furthermore, its pathogenesis is still poorly understood.
To determine the T-cell phenotypes involved in nasal polyposis in Chinese patients.
Twenty-four Chinese patients with nasal polyps were studied. CD4, CD8, Foxp3, and interleukin (IL) 17 were analyzed by immunohistochemical staining. Expression of T-bet, GATA-3, Foxp3, and RORgammat mRNA was detected by real-time polymerase chain reaction. The levels of T-cell cytokines (IL-4, IL-5, interferon [IFN] gamma, IL-10, IL-17, and transforming growth factor [TGF] beta) were determined using enzyme-linked immunosorbent assay, and serum immunoglobulin (Ig) E levels were measured using the UNICAP system.
Increased expression of CD4+ and CD8+ and decreased expression of Foxp3 and IL-17 were detected in nasal polyps compared with control tissue. Furthermore, expression of T-bet and GATA-3 mRNA was upregulated, whereas Foxp3 mRNA expression was markedly downregulated. Furthermore, increased levels of IFN-gamma, IL-4 and IL-5 and decreased levels of IL-10 and TGF-beta were found in nasal polyps. There was no association between Staphylococcus aureus exotoxin (SAE)-specific IgE and T regulatory cell (Treg) insufficiency in nasal polyps.
Our findings demonstrate that excessive infiltration of CD4+ and CD8+ T cells in nasal polyps may be associated with expression of Foxp3+ by Tregs but not with SAEs in Chinese patients.
鼻息肉在西方国家和东方国家有着不同的病因。此外，其发病机制仍未被充分了解。
确定中国患者鼻息肉中涉及的T细胞表型。
对24例中国鼻息肉患者进行研究。通过免疫组织化学染色分析CD4、CD8、Foxp3和白细胞介素（IL）-17。采用实时聚合酶链反应检测T-bet、GATA-3、Foxp3和RORγt mRNA的表达。使用酶联免疫吸附测定法测定T细胞细胞因子（IL-4、IL-5、干扰素[IFN]γ、IL-10、IL-17和转化生长因子[TGF]β）的水平，并使用UNICAP系统测量血清免疫球蛋白（Ig）E水平。
与对照组织相比，鼻息肉中检测到CD4+和CD8+表达增加，Foxp3和IL-17表达减少。此外，T-bet和GATA-3 mRNA表达上调，而Foxp3 mRNA表达明显下调。此外，鼻息肉中IFN-γ、IL-4和IL-5水平升高，IL-10和TGF-β水平降低。鼻息肉中金黄色葡萄球菌外毒素（SAE）特异性IgE与T调节细胞（Treg）功能不全之间无关联。
我们的研究结果表明，中国患者鼻息肉中CD4+和CD8+ T细胞的过度浸润可能与Treg的Foxp3+表达有关，而与SAE无关。
