Zhang Chaofeng, Zhou Aichun, Zhang Mian
Key Laboratory of Modern Chinese Medicines, Ministry of Education, China Pharmaceutical University, Nanjing 210038, China.
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(8):994-8.
To investigate the chemical constituents with immunosuppressive function from Alisma orientalis.
The chemical constituents were isolated and purified by kinds of column chromatography and its structures were elucidated by NMR spectra and physicochemical properties. Its immunocompentence of lymphocytes taken from spleen of mouse were examined by MTT assay.
Twelve compounds were isolated and identified as clovandiol (1), orientalol E (2), alismoxide (3), alismol (4), 4alpha, l0alpha-dihydroxy-5beta-H-guaj-6-en (5), alismorientols A (6), alisol F (7), alisol A (8), 13beta,17beta-epoxy alisol A (9), alisol B 23-acetate (10), 1H-indole-3-carboxylic acid (11) and cuccinic acid (12). Compounds 9, 10 and alisol A 24-acetate showed immunosuppressive function.
Compounds 1, 5, 11 and 12 were isolated firstly from this genus,and the NMR spectra data of 1 were corrected firstly, some protostan-type triterpenoids may be developed as new drug with immunosuppressive function.
研究泽泻中具有免疫抑制功能的化学成分。
采用多种柱色谱法对化学成分进行分离纯化，并通过核磁共振光谱和理化性质对其结构进行鉴定。采用MTT法检测从小鼠脾脏中提取的淋巴细胞的免疫活性。
分离鉴定出12个化合物，分别为异菖蒲二醇（1）、泽泻醇E（2）、泽泻氧化物（3）、泽泻醇（4）、4α,10α-二羟基-5β-H-愈创木-6-烯（5）、泽泻东方醇A（6）、泽泻醇F（7）、泽泻醇A（8）、13β,17β-环氧泽泻醇A（9）、泽泻醇B 23-乙酸酯（10）、1H-吲哚-3-羧酸（11）和琥珀酸（12）。化合物9、10和泽泻醇A 24-乙酸酯具有免疫抑制功能。
化合物1、5、11和12为首次从该属植物中分离得到，首次修正了化合物1的核磁共振光谱数据，一些原萜烷型三萜类化合物有望开发成为具有免疫抑制功能的新药。
