[芪参益气方对急性心肌梗死大鼠心脏治疗后心肌蛋白质组学变化的分析]
[Analysis of myocardial proteomic alteration after Qishenyiqi formula treatment in acute infarcted rat hearts].
作者信息
Hong Chengtao, Wang Yi, Lou Jianzhou, Liu Qian, Qu Haibin, Cheng Yiyu
机构信息
Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 310058, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(8):1018-21.
OBJECTIVE
Use proteomic technique to research the effects of Qishenyiqi formula in acute myocardial infarcted rats, and further explore its protein-level mechanism.
METHOD
The rat hearts of sham, myocardial infarcted and Qishenyiqi formula treated groups were collected. Analyzing and comparing the differences of protein expressions in each groups with two-dimensional electrophoresis, computer-assisted image analysis and matrix-assisted laser desorption/ionization mass spectrometry.
RESULT
Compared with myocardial infarcted group, there were 14 protein expressions changed in Qishenyiqi formula treated group. The result showed that 9 protein expressions were up-regulated, and 5 ones were down-regulated significantly. These proteins are associated with energy metabolism, mitochondrial function, oxidative stress, cytoskeleton and others.
CONCLUSION
Myocardial protective effects of Qishenyiqi formula in myocardial infarcted procedure might be closely related to the recovery of energy supply, the reduction of oxidative stress, as well as the promotion of cell recovery.
目的
运用蛋白质组学技术研究芪参益气方对急性心肌梗死大鼠的影响，并进一步探讨其在蛋白质水平的作用机制。
方法
收集假手术组、心肌梗死组和芪参益气方治疗组大鼠的心脏。采用二维电泳、计算机辅助图像分析和基质辅助激光解吸/电离质谱分析比较各组蛋白质表达的差异。
结果
与心肌梗死组相比，芪参益气方治疗组有14种蛋白质表达发生变化。结果显示，9种蛋白质表达上调，5种蛋白质表达显著下调。这些蛋白质与能量代谢、线粒体功能、氧化应激、细胞骨架等相关。
结论
芪参益气方在心肌梗死过程中的心肌保护作用可能与能量供应的恢复、氧化应激的降低以及细胞恢复的促进密切相关。
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