School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
J Ethnopharmacol. 2010 Oct 5;131(3):575-80. doi: 10.1016/j.jep.2010.07.036. Epub 2010 Jul 24.
Shuanglong Formula (SLF) is a classic formula for treating heart disease in traditional Chinese medicine (TCM). And it has been reported that the combinational treatment of SLF with autologous mesenchymal stem cells (MSCs) transplantation could be of benefit to people with acute myocardial infarction. However, their underlying mechanisms are not clear.
The effects of whole formula and its herbal main ingredients on rat MSCs differentiation towards cardiomyocytes were investigated and the distinct protein expression profile in MSC-derived cardiomyocytes studied.
The protein expression profiles of rat MSCs and cardiomyocyte-like cells were determined with two-dimensional gel electrophoresis (2-DE).
Our data demonstrated that SLF can induce MSCs into cardiomyocyte-like cells and around 36 proteins mainly involved in cytoskeleton, cell tissue energy metabolism and signal transduction have been shown to be regulated distinctly by SLF treatment.
Data presented in this study suggest that large rearrangement of the proteome occur during the differentiation process of the MSCs to terminally differentiated cardiomyocyte-like cells and offer the possibility for further characterization of specific targets driving the stem cell differentiation.
双龙方（SLF）是一种治疗中医心脏病的经典方剂。据报道，SLF 联合自体间充质干细胞（MSCs）移植的联合治疗对急性心肌梗死患者有益。然而，其潜在机制尚不清楚。
研究全方及其草药主要成分对大鼠 MSCs 向心肌细胞分化的影响，并研究 MSC 衍生的心肌细胞中的独特蛋白表达谱。
采用二维凝胶电泳（2-DE）测定大鼠 MSCs 和心肌样细胞的蛋白表达谱。
我们的数据表明，SLF 可诱导 MSCs 向心肌样细胞分化，并且大约 36 种主要涉及细胞骨架、细胞组织能量代谢和信号转导的蛋白质被证明通过 SLF 处理而得到明显调节。
本研究提供的数据表明，在 MSCs 向终末分化的心肌样细胞分化过程中，蛋白质组发生了大规模重排，并为进一步鉴定驱动干细胞分化的特定靶标提供了可能。
