Dipartimento di Sanita Pubblica Veterinaria e Sicurezza Alimentare, Istituto Superiore di Sanita, Viale Regina Elena 299, Roma, Italy.
J Agric Food Chem. 2009 Aug 26;57(16):7200-4. doi: 10.1021/jf901181m.
Salmonella is one of the main organisms causing outbreaks of foodborne illness, and meat is one of the major vehicles of salmonellosis throughout the world. A novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (ELIME array), is proposed for the detection of Salmonella in meat samples. After an optimization study, using Salmonella enterica serotype Enteritidis as reference antigen, the ability of the method to interact with a large number of Salmonella serovars commonly present in food was evaluated. The assay was then used to analyze samples of pork, chicken, beef, and turkey experimentally inoculated with Salmonella as well as real samples. The results were compared with those from the International Standard of Organization (ISO) culture method. The comparison showed that the ELIME array is able to detect a low number of Salmonella cells (1-10 CFU per 25 g) after only 6 h of incubation in a pre-enrichment broth. The investigation revealed a very good agreement between culture and ELIME array methods for meat samples, reducing the time for performing the analysis and obtaining the results quickly.
沙门氏菌是引起食源性疾病暴发的主要病原体之一，而肉类是全球沙门氏菌病的主要传播媒介之一。本研究提出了一种新型的基于 96 孔电化学板与免疫磁珠偶联的分析免疫传感器阵列（ELIME 阵列），用于检测肉样中的沙门氏菌。经过优化研究，以肠炎沙门氏菌血清型为参考抗原，评估了该方法与食品中常见的大量沙门氏菌血清型相互作用的能力。然后，该方法用于分析经沙门氏菌人工接种的猪肉、鸡肉、牛肉和火鸡样本以及实际样本。将结果与国际标准化组织（ISO）培养方法进行比较。结果表明，在预增菌肉汤中孵育 6 小时后，ELIME 阵列仅能检测到低数量的沙门氏菌细胞（每 25 克 1-10 CFU）。研究表明，对于肉类样本，培养法和 ELIME 阵列法之间具有非常好的一致性，可缩短分析时间，快速获得结果。
